Molecular epidemiology of Mycobacterium bovis in Cameroon.
Molecular epidemiology of Mycobacterium bovis in Cameroon.
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DOI：
10.1038/s41598-017-04230-6
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发表时间：
2017-07-05
影响因子：
4.6
通讯作者：
de C Bronsvoort BM
中科院分区：
文献类型：
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作者：
Egbe NF;Muwonge A;Ndip L;Kelly RF;Sander M;Tanya V;Ngwa VN;Handel IG;Novak A;Ngandalo R;Mazeri S;Morgan KL;Asuquo A;de C Bronsvoort BM
我们描述了撒哈拉以南非洲国家最大规模的牛结核病分子流行病学研究，具有更高的空间分辨率，提供了对牛结核病的新见解。从2012年至2013年在巴门达、恩冈代雷、加鲁阿和马鲁阿屠宰场屠宰的2,346头牛中，分别采集了201头和179头有和没有牛结核样病变的牛的样本进行培养。用缺失分析、Hain®基因分型、SPOGO分型和MIRU-VNTR法对255株牛分枝杆菌进行分离、鉴定和基因分型。非洲1号是主要的牛分枝杆菌克隆复合体，有97种独特的基因类型，包括19个新的种子型，代表了迄今为止在非洲观察到的最高的牛分枝杆菌遗传多样性。观察到的孢子型以SB0944和SB0953为主(63%)。有多个病变的动物中有三分之一有多个菌株感染。与西北地区相比，阿达马瓦地区牛分枝杆菌的多样性较高，但几乎没有近期传播的证据。与西北地区相比，阿达马瓦地区独生子女的频率很高，这可能是因为活跃的牲畜流动网络不断增加，而西北地区的当地扩张更为明显。后者与基于人口的推论相结合，表明西北地区和阿达马瓦地区分别处于不稳定和稳定的结核病流行状态。
We describe the largest molecular epidemiological study of Bovine Tuberculosis (bTB) in a sub-Saharan African country with higher spatial resolution providing new insights into bTB. Four hundred and ninety-nine samples were collected for culture from 201 and 179 cattle with and without bTB-like lesions respectively out of 2,346 cattle slaughtered at Bamenda, Ngaoundere, Garoua and Maroua abattoirs between 2012–2013. Two hundred and fifty-five M. bovis were isolated, identified and genotyped using deletion analysis, Hain® Genotype MTBC, spoligotyping and MIRU-VNTR. African 1 was the dominant M. bovis clonal complex, with 97 unique genotypes including 19 novel spoligotypes representing the highest M. bovis genetic diversity observed in Africa to date. SB0944 and SB0953 dominated (63%) the observed spoligotypes. A third of animals with multiple lesions had multiple strain infections. Higher diversity but little evidence of recent transmission of M. bovis was more common in Adamawa compared to the North-West Region. The Adamawa was characterised by a high frequency of singletons possibly due to constant additions from an active livestock movement network compared to the North-West Region where a local expansion was more evident. The latter combined with population-based inferences suggest an unstable and stable bTB-endemic status in the North-West and Adamawa Regions respectively.
影响因子：
3.7
作者：
Tanaka MM;Phong R;Francis AR
通讯作者：
Francis AR