Kinetic analysis of the interactions between plant thioredoxin and target proteins.
Kinetic analysis of the interactions between plant thioredoxin and target proteins.
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DOI：
10.3389/fpls.2013.00508
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发表时间：
2013
影响因子：
5.6
通讯作者：
Hisabori T
中科院分区：
文献类型：
--
作者：
Hara S;Hisabori T
硫氧还蛋白是一种关键蛋白质，它在体内介导还原当量的转移，并在几种情况下调节氧化还原敏感酶。此外，硫氧还蛋白提供了氧化还原酶的还原等价物，如过氧氧化还蛋白。通过二硫醇-二硫键交换反应，还原形式的硫氧还蛋白优先与氧化形式的靶相互作用，氧化形式的靶在反应完成后立即释放。为了更全面地研究硫氧还蛋白与其靶蛋白之间的相互作用，我们合成了一个缺失第二个半胱氨酸的硫氧还蛋白突变体，并用表面等离子体共振技术监测了它们之间的相互作用。硫氧还蛋白与其靶标的结合率根据靶标使用还原等价物的不同而有很大不同：受分子中半胱氨酸对(S)还原或氧化控制活性的酶，以及使用硫氧还蛋白提供的还原等价物进行催化的酶。此外，硫氧还蛋白显示出对氧化靶标的更强偏好。这些结果解释了硫氧还蛋白与氧化目标选择性结合的原因，而当二硫醇-二硫键交换反应完成时，硫氧还蛋白与还原目标立即解离。
Thioredoxin is a critical protein that mediates the transfer of reducing equivalents in vivo and regulates redox sensitive enzymes in several cases. In addition, thioredoxin provides reducing equivalents to oxidoreductases such as peroxiredoxin. Through a dithiol–disulfide exchange reaction, the reduced form of thioredoxin preferentially interacts with the oxidized forms of targets, which are immediately released after this reaction is complete. In order to more thoroughly characterize these interactions between thioredoxin and its target proteins, a mutant version of thioredoxin that lacked the second cysteine was synthesized and interactions were monitored by surface plasmon resonance. The binding rates of thioredoxin to its targets were very different depending on the use of reducing equivalents by the targets: the enzymes whose activity was controlled by reduction or oxidation of a cysteine pair(s) in the molecule and the enzymes that used reducing equivalents provided by thioredoxin for their catalysis. In addition, thioredoxin revealed a stronger preference for an oxidized target. These results explain the reason for selective association of thioredoxin with oxidized targets for reduction, whereas immediate dissociation from a reduced target when the dithiol–disulfide exchange reaction is complete.
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影响因子：
4.8
作者：
Collin, V;Issakidis-Bourguet, E;Miginiac-Maslow, M
通讯作者：
Miginiac-Maslow, M
影响因子：
27.5
作者：
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通讯作者：
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影响因子：
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Perkins A;Gretes MC;Nelson KJ;Poole LB;Karplus PA
通讯作者：
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影响因子：
4.8
作者：
Geck, MK;Hartman, FC
通讯作者：
Hartman, FC
影响因子：
3.9
作者：
Limauro, Danila;D'Ambrosio, Katia;Bartolucci, Simonetta
通讯作者：
Bartolucci, Simonetta