Mutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsis.
Mutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsis.
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SUMO E3 连接酶 SIZ1 突变破坏拟南芥成熟雌配子体
DOI：
10.1371/journal.pone.0029470
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lin J
中科院分区：
文献类型：
--
作者：
Ling Y;Zhang C;Chen T;Hao H;Liu P;Bressan RA;Hasegawa PM;Jin JB;Lin J
雌配子体是受精后产生胚和胚乳的多细胞单倍体结构，是研究细胞核迁移、细胞特化、细胞间通讯等过程的分子机制的理想模式系统。以前的研究发现，小泛素样修饰物（SUMO）E3连接酶，SIZ 1，参与了许多过程，这取决于特定的目标底物和抑制水杨酸（SA）的积累。在这里，我们报告SIZ 1介导生殖过程。SIZ 1在雌性器官中表达增强，但在花药或花粉中未检测到。在siz 1 -2母体来源的缺陷导致减少结实，无论植物内的SA浓度高。此外，苯胺蓝染色和扫描电镜显示，索和珠孔的花粉管引导在大小1 -2的植物被逮捕。FG 7期后，1 -2号胚珠的胚囊也迅速破裂。siz 1 -2突变对参与雌配子体发育或子房中花粉管引导的基因表达没有显著影响。总之，我们的研究结果表明，SIZ 1维持成熟雌配子体的稳定性和正常功能，这是必要的花粉管引导。
Female gametophyte is the multicellular haploid structure that can produce embryo and endosperm after fertilization, which has become an attractive model system for investigating molecular mechanisms in nuclei migration, cell specification, cell-to-cell communication and many other processes. Previous reports found that the small ubiquitin-like modifier (SUMO) E3 ligase, SIZ1, participated in many processes depending on particular target substrates and suppression of salicylic acid (SA) accumulation. Here, we report that SIZ1 mediates the reproductive process. SIZ1 showed enhanced expression in female organs, but was not detected in the anther or pollen. A defect in the siz1-2 maternal source resulted in reduced seed-set regardless of high SA concentration within the plant. Moreover, aniline blue staining and scanning electron microscopy revealed that funicular and micropylar pollen tube guidance was arrested in siz1-2 plants. Some of the embryo sacs of ovules in siz1-2 were also disrupted quickly after stage FG7. There was no significant affects of the siz1-2 mutation on expression of genes involved in female gametophyte development- or pollen tube guidance in ovaries. Together, our results suggest that SIZ1 sustains the stability and normal function of the mature female gametophyte which is necessary for pollen tube guidance.
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DOI：
10.1111/j.1365-313x.2007.03359.x
发表时间：
2008-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jin JB;Jin YH;Lee J;Miura K;Yoo CY;Kim WY;Van Oosten M;Hyun Y;Somers DE;Lee I;Yun DJ;Bressan RA;Hasegawa PM
通讯作者：
Hasegawa PM
影响因子：
56.9
作者：
Higashiyama, T;Yabe, S;Kuroiwa, T
通讯作者：
Kuroiwa, T
影响因子：
7.4
作者：
Cheong, Mi Sun;Park, Hyeong Cheol;Yun, Dae-Jin
通讯作者：
Yun, Dae-Jin
拟南芥 SUMO E3 连接酶 AtMMS21 是 NSE2/MMS21 的同源物，调节根部细胞增殖
影响因子：
7.2
作者：
Huang, Lixia;Yang, Songguang;Yang, Chengwei
通讯作者：
Yang, Chengwei
影响因子：
4.6
作者：
Clough, Emily;Moon, Woongjoon;Hazelrigg, Tulle
通讯作者：
Hazelrigg, Tulle