A sensitive electrochemical sensor for in vitro detection of parathyroid hormone based on a MoS(2)-graphene composite.
A sensitive electrochemical sensor for in vitro detection of parathyroid hormone based on a MoS(2)-graphene composite.
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DOI：
10.1038/srep34587
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发表时间：
2016-10-03
影响因子：
4.6
通讯作者：
Kim T
中科院分区：
文献类型：
--
作者：
Kim HU;Kim HY;Kulkarni A;Ahn C;Jin Y;Kim Y;Lee KN;Lee MH;Kim T
本文报道了一种基于二硫化钼-石墨烯（MG）复合材料的生物传感器，可以测量患者血清样品中的甲状旁腺激素（PTH）浓度。通过电化学传感技术分析PTH和MG之间的相互作用。使用l-半胱氨酸将MG官能化。在此之后，PTH可以共价固定在MG传感电极上。利用扫描电子显微镜、高分辨透射电子显微镜、X射线衍射、拉曼光谱、X射线光电子能谱和傅里叶变换红外光谱对MG的性能进行了评价。优化后的固定化材料，如MG，PTH和碱性磷酸酶（ALP）的MG传感器的性能进行了研究，通过循环伏安法，以评估其线性，重复性和再现性。在氧化石墨烯（GO）和MG修饰的电极上进行电化学阻抗谱，以确认靶向PTH的单克隆抗体（MAb）的捕获。此外，ALP-PTH-MG传感器在1-50 pg mL-1的范围内对来自人工血清的PTH表现出线性响应。此外，使用ALP-PTH-MG传感器评估患者血清（n = 30），并使用标准设备（Roche E 170）进行比较。当使用Welch校正进行t检验时，P值小于0.01。这意味着所制造的传感器可以用于医疗诊断。
This paper reports a biosensor based on a MoS2-graphene (MG) composite that can measure the parathyroid hormone (PTH) concentration in serum samples from patients. The interaction between PTH and MG was analysed via an electrochemical sensing technique. The MG was functionalized using l-cysteine. Following this, PTH could be covalently immobilized on the MG sensing electrode. The properties of MG were evaluated using scanning electron microscopy, high-resolution transmission electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectrometry. Following optimization of immobilized materials—such as MG, PTH, and alkaline phosphatase (ALP)—the performance of the MG sensor was investigated via cyclic voltammetry, to assess its linearity, repeatability, and reproducibility. Electrochemical impedance spectroscopy was performed on graphene oxide (GO) and MG-modified electrodes to confirm the capture of a monoclonal antibody (MAb) targeting PTH. Furthermore, the ALP-PTH-MG sensor exhibits a linear response towards PTH from artificial serum over a range of 1–50 pg mL−1. Moreover, patient sera (n = 30) were evaluated using the ALP-PTH-MG sensor and compared using standard equipment (Roche E 170). The P-value is less than 0.01 when evaluated with a t-test using Welch’s correction. This implies that the fabricated sensor can be deployed for medical diagnosis.
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影响因子：
15
作者：
HUMMERS, WS;OFFEMAN, RE
通讯作者：
OFFEMAN, RE
影响因子：
10.9
作者：
Su, Yuezeng;Zhang, Yi;Feng, Xinliang
通讯作者：
Feng, Xinliang
DOI：
10.1016/j.btre.2014.06.003
发表时间：
2014-09
期刊：
Biotechnology reports (Amsterdam, Netherlands)
影响因子：
--
作者：
Kim HY;Jang KJ;Veerapandian M;Kim HC;Seo YT;Lee KN;Lee MH
通讯作者：
Lee MH
影响因子：
4.9
作者：
Chang, Kun;Chen, Weixiang
通讯作者：
Chen, Weixiang
影响因子：
29.4
作者：
Prabakar, S. J. Richard;Hwang, Yun-Hwa;Pyo, Myoungho
通讯作者：
Pyo, Myoungho