Adrenocorticotropin and the time of day both influence the amount and activity of 3-hydroxy-3-methylglutaryl coenzyme-A reductase in the hamster adrenal.
Adrenocorticotropin and the time of day both influence the amount and activity of 3-hydroxy-3-methylglutaryl coenzyme-A reductase in the hamster adrenal.
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促肾上腺皮质激素和一天中的时间都会影响仓鼠肾上腺中 3-羟基-3-甲基戊二酰辅酶-A 还原酶的数量和活性。
作者：
J. Lehoux;A. Lefèbvre;S. Bélisle;D. Bellabarba
研究了ACTH单独或与放线菌酮合用对金黄地鼠肾上腺3-羟基-3-甲基戊二酰辅酶A(HMG-CoA)还原酶蛋白含量和活性的影响。用兔抗大鼠肝还原酶抗体和125I标记的蛋白A对匀浆和微粒体进行免疫印迹。ACTH可引起HMG-CoA还原酶蛋白含量和活性的剂量依赖性增加。一项时程研究显示，这种反应的潜伏期为60分钟，随后在120分钟和180分钟时显著增加。在180分钟时，微粒体还原酶活性显著提高4.7倍，而微粒体和匀浆制剂的还原酶蛋白质含量分别提高4.4倍和3.1倍。我们计算出只有40%-50%的还原酶蛋白质含量是微粒体，这表明肾上腺中存在不止一个酶池。放线菌亚胺与ACTH合用不仅可以阻止激素对蛋白质含量和还原酶活性的影响，而且在处理180min后，还原酶的蛋白质和活性分别下降58-69%和65-86%。此外，还原酶活性和蛋白质含量在白天也是平行波动的。在蛋白水解酶抑制剂和碘乙酰胺存在下，免疫印迹显示在102K摩尔重量区有一条主带。这些结果表明，ACTH和其他引起肾上腺HMG-CoA还原酶活性变化的条件也同时引起还原酶蛋白含量的变化。该还原酶的基本单位的表观摩尔质量约为102K。
We studied the effect of ACTH, alone or in combination with cycloheximide, on hamster adrenal 3-hydroxy-3-methylglutaryl coenzyme-A (HMG-CoA) reductase protein content (mass) and activity. Immunoblotting was performed on both homogenate and microsomal preparations, using a rabbit antirat liver reductase antibody and 125I-labeled protein A. A dose-dependent increase in HMG-CoA reductase protein content and activity was produced by ACTH. A time-course study revealed a latency of 60 min, followed by a significant increase at 120 and 180 min, in this response. At 180 min, microsomal reductase activity was significantly increased 4.7-fold, whereas the reductase protein content of microsomes and homogenate preparations was enhanced 4.4- and 3.1-fold, respectively. We calculated that only 40-50% of the reductase protein content was microsomal, indicating that more than one pool of the enzyme is present in adrenals. The coadministration of cycloheximide with ACTH not only prevented the hormonal effect on the protein content and activity of the reductase, but also produced, 180 min posttreatment, 58-69% and 65-86% decreases in reductase protein and activity, respectively. Also, both reductase activity and protein content fluctuated in parallel during the day. In the presence of proteolytic enzyme inhibitors and iodoacetamide, a major band in the area of 102K mol wt was evidenced by immunoblotting. These results indicate that ACTH and other conditions that provoke a change in adrenal HMG-CoA reductase activity also induce, in parallel, a change in the reductase protein content. The basic unit of the reductase has an apparent mol wt of about 102K.
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DOI：
--
发表时间：
1985-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Ness;M. J. Mccreery;C. Sample;M. Smith;L. Pendleton
通讯作者：
G. Ness;M. J. Mccreery;C. Sample;M. Smith;L. Pendleton
牛肾上腺皮质细胞原代培养物中 ACTH 诱导 3-羟基-3-甲基戊二酰辅酶 A 还原酶、胆固醇生物合成和类固醇生成。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rainey,WE;Shay,JW;Mason,JI
通讯作者：
Mason,JI
影响因子：
4.8
作者：
Gao, Yansong;Zhou, Yulian;Radhakrishnan, Arun
通讯作者：
Radhakrishnan, Arun
DOI：
10.1016/s0021-9258(18)90983-x
发表时间：
1984-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Clarke;A. Fogelman;P. Edwards
通讯作者：
C. Clarke;A. Fogelman;P. Edwards
抗康克汀 C100 细胞中 3-羟基-3-甲基戊二酰辅酶 A 还原酶的 Mr 92,000 原体过量产生。
DOI：
10.1073/pnas.80.6.1516
发表时间：
1983
影响因子：
11.1
作者：
Hardeman,EC;Jenke,HS;Simoni,RD
通讯作者：
Simoni,RD