Asymmetric distribution of cadherin 23 and protocadherin 15 in the kinocilial links of avian sensory hair cells.
Asymmetric distribution of cadherin 23 and protocadherin 15 in the kinocilial links of avian sensory hair cells.
复制标题
DOI：
10.1002/cne.22456
复制
发表时间：
2010-11-01
影响因子：
2.5
通讯作者：
Richardson, Guy P.
中科院分区：
文献类型：
--
作者：
Goodyear, Richard J.;Forge, Andy;Legan, P. Kevin;Richardson, Guy P.
钙粘蛋白23和原钙粘蛋白15是顶端连接的组成部分，顶端连接是连接毛细胞静纤毛的细丝，据信可以控制毛细胞的机械传感器通道。尖端链接沿着毛束的机械敏感性轴对齐，从一个静纤毛的顶部倾斜地延伸到相邻的更高的静纤毛的侧面。在豚鼠听毛细胞中，尖端链接被极化，其中钙粘蛋白23在上端，原钙粘蛋白15在下端，换能器通道位于此处。双免疫金标记的鸟类毛细胞被用来研究这两种蛋白质的分布在动纤毛链接，链接类型，重视最高的静纤毛的毛束动纤毛。在前庭毛束的动纤毛连接中，钙粘蛋白23定位于静纤毛，原钙粘蛋白15定位于动纤毛。因此，这两种钙粘蛋白在动纤毛链内不对称地分布，但具有极性，即，在沿沿着毛束的敏感轴对齐的那些链内，相对于尖端链的极性相反。在单宁酸存在下固定的毛束的常规透射电子显微镜揭示了位于离动纤毛膜35-40 nm的120-130 nm长的动纤毛链接中的明显密度。该密度的位置与其是在钙粘蛋白23和原钙粘蛋白15的同型二聚体形式的相对N-末端之间以反式构型发生相互作用的位点一致。J. Comp.神经元518：4288-4297，2010.© 2010 Wiley-Liss公司。
Cadherin 23 and protocadherin 15 are components of tip links, fine filaments that interlink the stereocilia of hair cells and are believed to gate the hair cell's mechanotransducer channels. Tip links are aligned along the hair bundle's axis of mechanosensitivity, stretching obliquely from the top of one stereocilium to the side of an adjacent, taller stereocilium. In guinea pig auditory hair cells, tip links are polarized with cadherin 23 at the upper end and protocadherin 15 at the lower end, where the transducer channel is located. Double immunogold labeling of avian hair cells was used to study the distribution of these two proteins in kinocilial links, a link type that attaches the tallest stereocilia of the hair bundle to the kinocilium. In the kinocilial links of vestibular hair bundles, cadherin 23 localizes to the stereocilium and protocadherin 15 to the kinocilium. The two cadherins are therefore asymmetrically distributed within the kinocilial links but of a polarity that is, within those links that are aligned along the hair bundle's axis of sensitivity, reversed relative to that of tip links. Conventional transmission electron microscopy of hair bundles fixed in the presence of tannic acid reveals a distinct density in the 120–130 nm long kinocilial links that is located 35–40 nm from the kinociliary membrane. The location of this density is consistent with it being the site at which interactions occur in an in trans configuration between the opposing N-termini of homodimeric forms of cadherin 23 and protocadherin 15. J. Comp. Neurol. 518:4288–4297, 2010. © 2010 Wiley-Liss, Inc.
登录
查看更多内容
DOI：
10.1073/pnas.2334417100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Belyantseva, IA;Boger, ET;Friedman, TB
通讯作者：
Friedman, TB
影响因子：
25
作者：
Petit, Christine;Richardson, Guy P.
通讯作者：
Richardson, Guy P.
影响因子：
56.9
作者：
Sidi, S;Friedrich, RW;Nicolson, T
通讯作者：
Nicolson, T
影响因子：
2.7
作者：
Lagziel, A;Ahmed, ZM;Friedman, TB
通讯作者：
Friedman, TB
影响因子：
5.3
作者：
Michalski, Nicolas;Michel, Vincent;Petit, Christine
通讯作者：
Petit, Christine