Identification, characterization, and isolation of a common progenitor for osteoclasts, macrophages, and dendritic cells from murine bone marrow and periphery.
Identification, characterization, and isolation of a common progenitor for osteoclasts, macrophages, and dendritic cells from murine bone marrow and periphery.
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DOI：
10.1002/jbmr.1822
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发表时间：
2013-05
影响因子：
6.2
通讯作者：
Aguila, Hector Leonardo
中科院分区：
文献类型：
--
作者：
Jacome-Galarza, Christian E.;Lee, Sun-Kyeong;Lorenzo, Joseph A.;Aguila, Hector Leonardo
破骨细胞是来源于单核细胞前体的特化骨吸收细胞。我们已经在小鼠骨髓中鉴定出三种具有高破骨细胞生成潜能的细胞群，它们表达的表型为：B220− CD 3 − CD 11b −/低CD 115+和CD 117 hi、CD 117 intermediate或CD 117 low。我们已经评估了这些群体产生巨噬细胞和树突状细胞的能力。在单个细胞水平上，表达较高CD 117水平的群体能够在体外产生骨吸收破骨细胞，吞噬巨噬细胞和抗原呈递树突状细胞，效率超过90%，表明这些细胞类型存在共同的发育途径。具有破骨细胞生成潜能的细胞也存在于血液和外周造血器官中。它们的功能意义和/或它们与骨髓祖细胞的关系尚未完全确定。因此，我们的特点是小鼠外周细胞群体的能力，形成破骨细胞，巨噬细胞和树突状细胞在体外。脾脏和外周血单核细胞祖细胞与骨髓祖细胞具有相同的表型标志物，但其CD 11b的表达不同，其在骨髓中低而在外周中高。我们建议，循环单核细胞祖细胞来自一个共同的骨髓破骨细胞/巨噬细胞/树突状细胞祖细胞（OcMDC），我们现在已经在克隆水平的特点。然而，骨髓和外周血单核细胞祖细胞之间的谱系关系尚未确定。
Osteoclasts are specialized bone resorbing cells that derive from monocyte precursors. We have identified three populations of cells with high osteoclastogenic potential in murine bone marrow, which expressed the phenotype: B220−CD3−CD11b−/low CD115+ and either CD117hi, CD117intermediate or CD117low. We have evaluated these populations for their ability to also generate macrophages and dendritic cells. At a single cell level, the population expressing higher CD117 levels was able to generate bone-resorbing osteoclasts, phagocytic macrophages and antigen-presenting dendritic cells in vitro with efficiencies of over 90 percent, indicating that there exists a common developmental pathway for these cell types. Cells with osteoclastogenic potential also exist in blood and peripheral hematopoietic organs. Their functional meaning and/or their relationship with bone marrow progenitors is not well established. Hence, we characterized murine peripheral cell populations for their ability to form osteoclasts, macrophages and dendritic cells in vitro. The spleen and peripheral blood monocyte progenitors share phenotypic markers with bone marrow progenitors, but differ in their expression of CD11b, which was low in bone marrow but high in periphery. We propose that circulating monocyte progenitors are derived from a common bone marrow osteoclasts/macrophage/dendritic cell progenitor (OcMDC), which we have now characterized at a clonal level. However, the lineage relationship between the bone marrow and peripheral monocyte progenitors has yet to be defined.
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DOI：
10.1084/jem.190.12.1741
发表时间：
1999-12-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Arai F;Miyamoto T;Ohneda O;Inada T;Sudo T;Brasel K;Miyata T;Anderson DM;Suda T
通讯作者：
Suda T
DOI：
10.1073/pnas.0401602101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Mócsai, A;Humphrey, MB;Nakamura, MC
通讯作者：
Nakamura, MC
影响因子：
56.9
作者：
Fogg, DK;Sibon, C;Geissmann, F
通讯作者：
Geissmann, F
影响因子：
6.2
作者：
Jacquin, C;Gran, DE;Aguila, HL
通讯作者：
Aguila, HL
影响因子：
20.3
作者：
Miyamoto, T;Ohneda, O;Suda, T
通讯作者：
Suda, T