Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China.
Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China.
复制标题
我国食用菌蜡样芽孢杆菌的鉴定及分子鉴定
DOI：
10.1186/s12866-020-01996-0
复制
发表时间：
2020-10-14
期刊：
影响因子：
4.2
通讯作者：
Ding Y
中科院分区：
文献类型：
--
作者：
Liu C;Yu P;Yu S;Wang J;Guo H;Zhang Y;Zhang J;Liao X;Li C;Wu S;Gu Q;Zeng H;Zhang Y;Wei X;Zhang J;Wu Q;Ding Y
背景
蜡样芽孢杆菌是一种常见于自然界和食物中的食源性病原体，可导致食物腐败和健康问题。虽然B.虽然世界各地都有关于食物中含有蜡状芽孢杆菌的报道，但食用菌作为传统或功能性食品越来越受欢迎，其受污染程度在很大程度上尚不清楚。本研究调查了B的流行情况、毒素基因分布、耐药性和遗传多样性。蜡状芽孢杆菌（cereus）。
结果
共采集食用菌样品699份，检出B污染样品198份，检出率为28.3%。蜡状芽孢杆菌的平均污染水平为55.4 MPN/g。247个B。从污染的样品中分离蜡样菌菌株。检测到7个肠毒素基因和1个催泪素合成酶基因。所有肠毒素基因的检出率均≥ 80%，而cesB基因在B.蜡样芽胞杆菌为3%。大多数分离株对青霉素类、β-内酰胺/β-内酰胺酶抑制剂组合、头孢烯类和安莎霉素类耐药，但对青霉烯类、氨基糖苷类、大环内酯类、酮内酯类、糖肽类、喹诺酮类、苯丙醇类、四环素类、林可酰胺类、链阳性菌素类和硝基呋喃类敏感。同时，99.6%的菌株对三类或三类以上抗菌药物表现出多重耐药。利用遗传多样性分析，所有菌株被定义在171个序列类型（ST），其中83个菌株被分配到78个新的ST。
结论
这项研究提供了对B的患病率和潜在风险的大规模深入了解。中国食用菌中的蜡状芽孢杆菌。大约三分之一的样品被B污染。蜡样芽胞杆菌，几乎所有的菌株都表现出多重耐药性。7种肠毒素基因的检出率均≥ 80%。这些新发现可能表明需要对食用菌进行适当的前/后处理以消除B。蜡状芽孢杆菌，从而防止对公众健康的潜在风险。
Background
Bacillus cereus is a foodborne pathogen commonly found in nature and food and can cause food spoilage and health issues. Although the prevalence of B. cereus in foods has been reported worldwide, the extent of contamination in edible fungi, which has become increasingly popular as traditional or functional food, is largely unknown. Here we investigated the prevalence, toxin genes’ distribution, antibiotic resistance, and genetic diversity of B. cereus isolated from edible fungi in China.
Results
Six hundred and ninety-nine edible fungi samples were collected across China, with 198 (28.3%) samples found to be contaminated by B. cereus, with an average contamination level of 55.4 most probable number (MPN)/g. Two hundred and forty-seven B. cereus strains were isolated from the contaminated samples. Seven enterotoxin genes and one cereulide synthetase gene were detected. The detection frequencies of all enterotoxin genes were ≥ 80%, whereas the positive rate of the cesB gene in B. cereus was 3%. Most isolates were resistant to penicillins, β-lactam/β-lactamase inhibitor combinations, cephems, and ansamycins, but were susceptible to penems, aminoglycosides, macrolides, ketolide, glycopeptides, quinolones, phenylpropanol, tetracyclines, lincosamides, streptogramins, and nitrofurans. Meanwhile, 99.6% of all isolates displayed multiple antimicrobial resistance to three or more classes of antimicrobials. Using genetic diversity analysis, all isolates were defined in 171 sequence types (STs), of which 83 isolates were assigned to 78 new STs.
Conclusions
This study provides large-scale insight into the prevalence and potential risk of B. cereus in edible fungi in China. Approximately one-third of the samples were contaminated with B. cereus, and almost all isolates showed multiple antimicrobial resistance. Detection frequencies of all seven enterotoxin genes were equal to or more than 80%. These new findings may indicate a need for proper pre-/post-processing of edible fungi to eliminate B. cereus, thereby preventing the potential risk to public health.
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DOI：
10.1016/j.ijfoodmicro.2018.06.016
发表时间：
2018-11-02
影响因子：
5.4
作者：
Carter, Laurenda;Chase, Hannah R.;Gopinath, Gopal R.
通讯作者：
Gopinath, Gopal R.
影响因子：
19
作者：
Glasset, B.;Herbin, S.;Brisabois, A.
通讯作者：
Brisabois, A.
影响因子：
4.4
作者：
Hansen, BM;Hendriksen, NB
通讯作者：
Hendriksen, NB
影响因子：
3.1
作者：
BEECHER, DJ;MACMILLAN, JD
通讯作者：
MACMILLAN, JD
影响因子：
2.1
作者：
Ehling-Schulz, Monika;Guinebretiere, Marie-Helene;Svensson, Birgitta
通讯作者：
Svensson, Birgitta