Identification of Hemagglutinin Residues Responsible for H3N2 Antigenic Drift during the 2014-2015 Influenza Season.
Identification of Hemagglutinin Residues Responsible for H3N2 Antigenic Drift during the 2014-2015 Influenza Season.
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DOI：
10.1016/j.celrep.2015.06.005
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发表时间：
2015-07-07
期刊：
影响因子：
8.8
通讯作者：
Hensley SE
中科院分区：
文献类型：
--
作者：
Chambers BS;Parkhouse K;Ross TM;Alby K;Hensley SE
流感疫苗必须定期更新，因为流感病毒不断获得血凝素（HA）抗体结合位点的突变。2014-2015年流感季期间在北方半球流行的大多数H3 N2毒株与A/Texas/50/2012 H3 N2疫苗毒株抗原性不匹配。最近的H3 N2病毒株具有几种新的HA突变，目前尚不清楚这些突变中的哪一种导致了2014-2015年的疫苗错配。在这里，我们使用反向遗传学来证明HA抗原位点B的突变是导致当前错配的主要原因。从接种疫苗的人和感染的雪貂和绵羊中分离的血清具有降低的血凝抑制和体外中和滴度，其针对反向遗传学衍生的在HA抗原位点B中具有突变的病毒。这些数据为2014-2015年流感季节期间观察到的低流感疫苗效力提供了抗原性解释。此外，我们的数据支持世界卫生组织关于更新未来疫苗配方中H3 N2成分的决定。
Influenza vaccines must be updated regularly because influenza viruses continuously acquire mutations in antibody binding sites of hemagglutinin (HA). The majority of H3N2 strains circulating in the Northern Hemisphere during the 2014–2015 season are antigenically mismatched to the A/Texas/50/2012 H3N2 vaccine strain. Recent H3N2 strains possess several new HA mutations and it is unknown which of these mutations contribute to the 2014–2015 vaccine mismatch. Here, we use reverse-genetics to demonstrate that mutations in HA antigenic site B are primarily responsible for the current mismatch. Sera isolated from vaccinated humans and infected ferrets and sheep had reduced hemagglutination-inhibition and in vitro neutralization titers against reverse-genetics derived viruses possessing mutations in the HA antigenic site B. These data provide an antigenic explanation for the low influenza vaccine efficacy observed during the 2014–2015 influenza season. Further, our data support the World Health Organization’s decision to update the H3N2 component of future vaccine formulations.
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DOI：
10.1084/jem.20130212
发表时间：
2013-07-29
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Li Y;Myers JL;Bostick DL;Sullivan CB;Madara J;Linderman SL;Liu Q;Carter DM;Wrammert J;Esposito S;Principi N;Plotkin JB;Ross TM;Ahmed R;Wilson PC;Hensley SE
通讯作者：
Hensley SE
影响因子：
5.9
作者：
Yewdell, Jonathan W.
通讯作者：
Yewdell, Jonathan W.
影响因子：
64.8
作者：
WILEY, DC;WILSON, IA;SKEHEL, JJ
通讯作者：
SKEHEL, JJ
影响因子：
5.4
作者：
Abe, Y;Takashita, E;Hongo, S
通讯作者：
Hongo, S
影响因子：
5.4
作者：
Li, Yang;Bostick, David L.;Hensley, Scott E.
通讯作者：
Hensley, Scott E.