SARS-CoV-2 Is Not Detected in the Cerebrospinal Fluid of Encephalopathic COVID-19 Patients.
SARS-CoV-2 Is Not Detected in the Cerebrospinal Fluid of Encephalopathic COVID-19 Patients.
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DOI：
10.3389/fneur.2020.587384
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发表时间：
2020
影响因子：
3.4
通讯作者：
Lewis A
中科院分区：
文献类型：
--
作者：
Placantonakis DG;Aguero-Rosenfeld M;Flaifel A;Colavito J;Inglima K;Zagzag D;Snuderl M;Louie E;Frontera JA;Lewis A
新型冠状病毒SARS-CoV-2感染的神经系统表现受到广泛关注，但其机制仍不清楚。在这里，我们描述了来自公共领域RNA-seq数据集的计算数据和来自严重COVID-19肺炎成人患者的脑脊液数据，这些数据表明SARS-CoV-2感染中枢神经系统的可能性不大。我们发现，编码ACE 2受体和TMPRSS 2跨膜丝氨酸蛋白酶的mRNA，这两者都是病毒进入宿主细胞所必需的，在大脑的主要细胞类型中表达最低。此外，通过鼻咽拭子RT-PCR诊断为病毒感染的13名成人脑病COVID-19患者的CSF样本未显示病毒的证据。这个特别的发现有两个原因。首先，使用严格的标准验证了CSF研究的RT-PCR诊断;其次，这些患者中有61%在鼻咽诊断试验阳性的1周内进行了CSF检测。我们认为COVID-19的神经系统后遗症不是由于SARS-CoV-2脑膜脑炎，其他病因更可能是机制。
Neurologic manifestations of the novel coronavirus SARS-CoV-2 infection have received wide attention, but the mechanisms remain uncertain. Here, we describe computational data from public domain RNA-seq datasets and cerebrospinal fluid data from adult patients with severe COVID-19 pneumonia that suggest that SARS-CoV-2 infection of the central nervous system is unlikely. We found that the mRNAs encoding the ACE2 receptor and the TMPRSS2 transmembrane serine protease, both of which are required for viral entry into host cells, are minimally expressed in the major cell types of the brain. In addition, CSF samples from 13 adult encephalopathic COVID-19 patients diagnosed with the viral infection via nasopharyngeal swab RT-PCR did not show evidence for the virus. This particular finding is robust for two reasons. First, the RT-PCR diagnostic was validated for CSF studies using stringent criteria; and second, 61% of these patients had CSF testing within 1 week of a positive nasopharyngeal diagnostic test. We propose that neurologic sequelae of COVID-19 are not due to SARS-CoV-2 meningoencephalitis and that other etiologies are more likely mechanisms.
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影响因子：
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