Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging.
Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging.
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DOI：
10.1186/s12951-023-01859-6
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发表时间：
2023-04-01
影响因子：
10.2
通讯作者：
Lei, Ziqiang
中科院分区：
文献类型：
--
作者：
Guan, Xiaolin;Zhang, Liyuan;Lai, Shoujun;Zhang, Jiaming;Wei, Jingyu;Wang, Kang;Zhang, Wentao;Li, Chenghao;Tong, Jinhui;Lei, Ziqiang
关键词：
糖量子点（Glyco-quantum dots, glyco-QDs）在生物成像应用中引起了极大的兴趣，特别是在癌症成像中，因为它们有效地将糖簇效应与量子点的特殊光学特性结合在一起。目前的关键挑战在于如何消除传统的基于cd的有毒量子点对体内生物成像产生的高重金属毒性。本文报道了一种在水中通过巯基端单糖与金属盐前体“直接”反应制备无毒无cd糖量子点的生态友好途径。糖- cuins2量子点的形成可以用LaMer模型下的成核生长机制来解释。制备的4个糖- cuins2量子点均为水溶性、单分散、球形，尺寸范围为3.0 ~ 4.0 nm。它们在可见光区（500 ~ 590 nm）和近红外区（~ 827 nm）表现出分离良好的双发射，这可能是由于可见光激子发射和近红外表面缺陷发射所致。同时，细胞成像显示肿瘤细胞（HeLa, A549, MKN-45）具有明显的可逆双色（绿色和红色）荧光，并且基于其良好的生物识别能力，glyco-CuInS2量子点具有优异的膜靶向特性。重要的是，由于这些量子点具有高负电荷（zeta电位值范围为- 23.9至- 30.1 mV），因此能够均匀地穿透三维多细胞肿瘤球体（MCTS）的内部（坏死区），克服了现有量子点在体外球体模型中穿透深度差的问题。因此，共聚焦分析证实了它们出色的穿透和标记肿瘤的能力。因此，这些糖量子点在体内生物成像中的成功应用验证了这种设计策略是一种有效、低成本和简单的方法，可以开发绿色纳米颗粒作为廉价和有前途的荧光生物探针。在线版本包含补充材料，可在10.1186/s12951-023-01859-6获得。
Glyco-quantum dots (glyco-QDs) have attracted significant interest in bioimaging applications, notably in cancer imaging, because they effectively combine the glycocluster effect with the exceptional optical properties of QDs. The key challenge now lies in how to eliminate the high heavy metal toxicity originating from traditional toxic Cd-based QDs for in vivo bioimaging. Herein, we report an eco-friendly pathway to prepare nontoxic Cd-free glyco-QDs in water by the “direct” reaction of thiol-ending monosaccharides with metal salts precursors. The formation of glyco-CuInS2 QDs could be explained by a nucleation-growth mechanism following the LaMer model. As-prepared four glyco-CuInS2 QDs were water-soluble, monodispersed, spherical in shape and exhibited size range of 3.0–4.0 nm. They exhibited well-separated dual emission in the visible region (500–590 nm) and near-infrared range (~ 827 nm), which may be attributable to visible excitonic emission and near-infrared surface defect emission. Meanwhile, the cell imaging displayed the reversibly distinct dual-color (green and red) fluorescence in tumor cells (HeLa, A549, MKN-45) and excellent membrane-targeting properties of glyco-CuInS2 QDs based on their good biorecognition ability. Importantly, these QDs succeed in penetrating uniformly into the interior (the necrotic zone) of 3D multicellular tumor spheroids (MCTS) due to their high negative charge (zeta potential values ranging from − 23.9 to − 30.1 mV), which overcame the problem of poor penetration depth of existing QDs in in vitro spheroid models. So, confocal analysis confirmed their excellent ability to penetrate and label tumors. Thus, the successful application in in vivo bioimaging of these glyco-QDs verified that this design strategy is an effective, low cost and simple procedure for developing green nanoparticles as cheap and promising fluorescent bioprobes. The online version contains supplementary material available at 10.1186/s12951-023-01859-6.
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