A Method for Detecting Antioxidant Activity of Antioxidants by Utilizing Oxidative Damage of Pigment Protein
A Method for Detecting Antioxidant Activity of Antioxidants by Utilizing Oxidative Damage of Pigment Protein
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一种利用色素蛋白氧化损伤检测抗氧化剂抗氧化活性的方法
DOI：
10.1007/s12010-022-04058-5
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发表时间：
2022-07
影响因子：
3
通讯作者：
Hailun He
中科院分区：
文献类型：
--
作者：
CongLing Liu;Olena Zhur;XiaoTao Yan;TingTing Yin;HaiLian Rao;Xun Xiao;MingYang Zhou;CuiLing Wu;Hailun He
建立了一种简单有效的利用色素蛋白氧化损伤来检测其抗氧化活性的方法。该方法以藻蓝蛋白和牛血红蛋白为底物，AAPH为自由基引发剂，Trolox为阳性对照，植物乳杆菌793的发酵产物、藻蓝蛋白水解物、鲑鱼皮胶原蛋白水解物、合成肽PMRGGYHY和FCVLRP为本研究考察的抗氧化剂。由于受到自由基的攻击，色素蛋白在其特征吸收峰处的吸光度随时间变化。通过记录空白/阴性对照样品、Trolox阳性对照样品和受检抗氧化剂样品中色素蛋白的特征吸收峰随时间的变化曲线，可以利用特定的方程计算抗氧化剂的活性。藻蓝蛋白测定的线性范围为1~4μM，牛血红蛋白测定的线性范围为4~24μM。与ORAC法相比，该方法测得的样品的抗氧化活性略低。这项研究中提出的方法反映了所检测的抗氧化剂对色素蛋白的保护作用，这些抗氧化剂可能成为氧化损伤过程的新的生物标志物。
A simple and effective method for detecting the antioxidant activity by utilizing oxidative damage of pigment proteins was developed. In this method, phycocyanin and bovine hemoglobin pigment proteins were used as substrates attacked by free radicals; AAPH was used as a free radical initiator; and Trolox as a positive control; and the fermentation products of Lactobacillus plantarum 793, phycocyanin hydrolysates, salmon skin collagen hydrolysates, and synthetic peptides PMRGGYHY and FCVLRP are antioxidants inspected in this study. Because of being attacked by free radicals, the absorbance of the pigment proteins at their characteristic absorption peak changes with time. By recording the time-varying curve at the characteristic absorption peak of the pigment protein in the blank/negative control sample, the Trolox positive control sample, and the samples of inspected antioxidants, the antioxidant activity could be calculated by using the specific equation. The linear detection ranges of Trolox in the phycocyanin assay and the bovine hemoglobin assay were 1–4 μM and 4–24 μM, respectively. Compared with the ORAC assay, the antioxidant activities of the samples measured by this method were slightly lower. The method proposed in this study reflects the protective effects of inspected antioxidants on pigment proteins, which could potentially serve as new biomarkers of oxidative damage processes.
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影响因子：
2
作者：
R. Dmochowski
通讯作者：
R. Dmochowski
DOI：
10.1016/j.respe.2020.09.004
发表时间：
2020-10-10
期刊：
Revue D'Epidemiologie et De Sante Publique
影响因子：
--
作者：
La rédaction
通讯作者：
La rédaction
影响因子：
9.3
作者：
通讯作者：
--
影响因子：
5.9
作者：
Craighead FL
通讯作者：
Craighead FL