Sequence and transcriptional analysis of the genes responsible for curdlan biosynthesis in Agrobacterium sp. ATCC 31749 under simulated dissolved oxygen gradients conditions
Sequence and transcriptional analysis of the genes responsible for curdlan biosynthesis in Agrobacterium sp. ATCC 31749 under simulated dissolved oxygen gradients conditions
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农杆菌中负责凝胶多糖生物合成的基因的序列和转录分析。
DOI：
10.1007/s00253-011-3243-1
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发表时间：
2011-04
影响因子：
5
通讯作者：
Zhan, Xiaobei
中科院分区：
文献类型：
--
作者：
Wu, Jian-Rong;Jiang Yun;Zhang, Hong-Tao;Lin, Chi Chung;Yu, Xiao-Bing;Zheng, Zhi-Yong;Zhan, Xiaobei
农杆菌中 10 个选定基因的 mRNA 水平表达。通过 qRT-PCR 测定与电子转移链 (ETC)、三羧酸 (TCA) 循环、肽聚糖/脂多糖生物合成和尿苷二磷酸 (UDP)-葡萄糖生物合成相关的凝胶多糖发酵过程中各种溶解氧 (DO) 水平下的 ATCC 31749。实验在 DO 水平为 30%、50% 和 75% 以及低氧条件下进行。还研究了低氧条件下高细胞密度对上述基因转录反应的影响。除细胞色素 d (cyd A) 外，所有其他基因的转录水平在较高 DO 下均增加，并在 50% DO 时达到最大值。在 75% DO 下，所有基因的转录水平均受到抑制。此外，在低氧条件下的高细胞密度培养中，icd、sdh、cyo A和fix N基因的转录水平没有表现出显着波动。这些结果表明，在凝乳多糖发酵过程中，DO 通过调节 ETC、TCA 和 UDP-葡萄糖合成基因的转录水平来调节凝乳多糖合成。据我们所知，这是第一篇报道 DO 浓度明显调节农杆菌中的凝胶多糖生物合成。 ATCC 31749 为工业规模发酵的优化提供了必要的指导。
Expression at the mRNA level of ten selected genes in Agrobacterium sp. ATCC 31749 under various dissolved oxygen (DO) levels during curdlan fermentation related to electron transfer chain (ETC), tricarboxylic acid (TCA) cycle, peptidoglycan/lipopolysaccharide biosynthesis, and uridine diphosphate (UDP)-glucose biosynthesis were determined by qRT-PCR. Experiments were performed at DO levels of 30%, 50%, and 75%, as well as under low-oxygen conditions. The effect of high cell density on transcriptional response of the above genes under low oxygen was also studied. Besides cytochrome d (cyd A), the transcription levels of all the other genes were increased at higher DO and reached maximum at 50% DO. Under 75% DO, the transcriptional levels of all the genes were repressed. In addition, transcription levels of icd, sdh, cyo A, and fix N genes did not exhibit significant fluctuation with high cell density culture under low oxygen. These results suggested a mechanism for DO regulation of curdlan synthesis through regulation of transcriptional levels of ETCs, TCA, and UDP-glucose synthesis genes during curdlan fermentation. To our knowledge, this is the first report that DO concentration apparently regulates curdlan biosynthesis in Agrobacterium sp. ATCC 31749 providing essential lead for the optimization of the fermentation at the industrial scale.
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DOI：
10.1007/bf01576364
发表时间：
2005
期刊：
Journal of Industrial Microbiology
影响因子：
--
作者：
U. Rau;E. Gura;E. Olszewski;F. Wagner
通讯作者：
U. Rau;E. Gura;E. Olszewski;F. Wagner
影响因子：
3
作者：
Márcia Portilho;G. Matioli;G. Zanin;F. Moraes;A. Scamparini
通讯作者：
Márcia Portilho;G. Matioli;G. Zanin;F. Moraes;A. Scamparini
影响因子：
5
作者：
P. Verbelen;Sofie Saerens;S. E. V. Mulders;F. Delvaux;F. Delvaux
通讯作者：
P. Verbelen;Sofie Saerens;S. E. V. Mulders;F. Delvaux;F. Delvaux
影响因子：
2.8
作者：
T. Jarman;G. W. Pace
通讯作者：
T. Jarman;G. W. Pace
影响因子：
5
作者：
M. Trujillo-Roldán;S. Moreno;G. Espín;E. Galindo
通讯作者：
M. Trujillo-Roldán;S. Moreno;G. Espín;E. Galindo