RBCS1A and RBCS3B, two major members within the Arabidopsis RBCS multigene family, function to yield sufficient Rubisco content for leaf photosynthetic capacity.
RBCS1A and RBCS3B, two major members within the Arabidopsis RBCS multigene family, function to yield sufficient Rubisco content for leaf photosynthetic capacity.
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DOI：
10.1093/jxb/err434
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发表时间：
2012-03
影响因子：
6.9
通讯作者：
Ishida H
中科院分区：
文献类型：
--
作者：
Izumi M;Tsunoda H;Suzuki Y;Makino A;Ishida H
核酮糖1，5-二磷酸羧化酶/加氧酶（Rubisco）小亚基（RBCS）是由核内多基因家族编码的。RBCS多基因对Rubisco全酶积累和光合特性的贡献尚不清楚。从4个拟南芥RBCS基因中分离到RBCS 1A（rbcs 1a-1）和RBCS 3B（rbcs 3b-1）的T-DNA插入突变体，并得到一个双突变体（rbcs 1a 3b-1）。在rbcs 1a-1和rbcs 1a 3b-1中未检测到RBCS 1A mRNA，而在rbcs 3b-1和rbcs 1a 3b-1叶片中RBCS 3B mRNA水平被抑制至野生型水平的约20%。结果，总RBCS mRNA水平下降到52%，79%和23%的野生型水平rbcs 1a-1，rbcs 3b-1，和rbcs 1a 3b-1，分别。rbcs 1a-1、rbcs 3b-1和rbcs 1a 3b-1的Rubisco含量与总RBCS mRNA水平相似，Rubisco氮占总氮的比例分别为野生型的62%、78%和40%。RBCS 1A和RBCS 3B突变在rbcs 1a 3b-1中的作用明显是累加的。在环境CO2为40 Pa的CO2同化率降低Rubisco含量在各自的突变体叶片。虽然RBCS组成的Rubisco全酶的变化，每单位的Rubisco含量的CO2同化率是相同的基因型无关。这些结果清楚地表明，RBCS 1A和RBCS 3B有助于积累的Rubisco在拟南芥叶片中，这些基因的工作相加，以产生足够的光合能力的Rubisco。有人还建议，RBCS组合物中的Rubisco全酶不影响光合作用在目前的环境[CO2]条件下。
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit (RBCS) is encoded by a nuclear RBCS multigene family in many plant species. The contribution of the RBCS multigenes to accumulation of Rubisco holoenzyme and photosynthetic characteristics remains unclear. T-DNA insertion mutants of RBCS1A (rbcs1a-1) and RBCS3B (rbcs3b-1) were isolated among the four Arabidopsis RBCS genes, and a double mutant (rbcs1a3b-1) was generated. RBCS1A mRNA was not detected in rbcs1a-1 and rbcs1a3b-1, while the RBCS3B mRNA level was suppressed to ∼20% of the wild-type level in rbcs3b-1 and rbcs1a3b-1 leaves. As a result, total RBCS mRNA levels declined to 52, 79, and 23% of the wild-type level in rbcs1a-1, rbcs3b-1, and rbcs1a3b-1, respectively. Rubisco contents showed declines similar to total RBCS mRNA levels, and the ratio of Rubisco-nitrogen to total nitrogen was 62, 78, and 40% of the wild-type level in rbcs1a-1, rbcs3b-1, and rbcs1a3b-1, respectively. The effects of RBCS1A and RBCS3B mutations in rbcs1a3b-1 were clearly additive. The rates of CO2 assimilation at ambient CO2 of 40 Pa were reduced with decreased Rubisco contents in the respective mutant leaves. Although the RBCS composition in the Rubisco holoenzyme changed, the CO2 assimilation rates per unit of Rubisco content were the same irrespective of the genotype. These results clearly indicate that RBCS1A and RBCS3B contribute to accumulation of Rubisco in Arabidopsis leaves and that these genes work additively to yield sufficient Rubisco for photosynthetic capacity. It is also suggested that the RBCS composition in the Rubisco holoenzyme does not affect photosynthesis under the present ambient [CO2] conditions.
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