Co-crystallization of an ETS Domain (PU.1) in Complex with DNA
Co-crystallization of an ETS Domain (PU.1) in Complex with DNA
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ETS 结构域 (PU.1) 与 DNA 复合物的共结晶
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
K. R. Ely
中科院分区：
文献类型：
--
作者：
F. Pio;C. Ni;R. Mitchell;J. Knight;S. McKercher;M. Klemsz;A. Lombardo;R. Maki;K. R. Ely
PU.1转录因子是调节蛋白的ets基因家族的成员。这些分子在正常发育中发挥作用，并且还涉及恶性过程，例如红细胞白血病的发展。Ets蛋白共有一个保守的DNA结合结构域（ETS结构域），该结构域识别具有核心序列5′-C/AGGAA/T-3′的富含嘌呤的序列。与许多其他DNA结合蛋白不同，该结构域作为单体与DNA结合。PU.1转录因子的ETS结构域已与含有识别序列的16个碱基对的寡核苷酸复合结晶。形成的晶体空间群为C2，a = 89.1，B = 101.9，c = 55.6，β = 111.2°，且至少为2.3。在不对称单元中有两个络合物。大的可用晶体的生产取决于蛋白质和DNA组分的长度，在末端使用具有未配对的A和T碱基的寡核苷酸，以及结晶溶液中聚乙二醇和乙酸锌的存在。这是第一个被结晶的ETS结构域，用于结晶该复合物的策略可能对ETS家族的其他成员有用。
The PU.1 transcription factor is a member of the ets gene family of regulatory proteins. These molecules play a role in normal development and also have been implicated in malignant processes such as the development of erythroid leukemia. The Ets proteins share a conserved DNA-binding domain (the ETS domain) that recognizes a purine-rich sequence with the core sequence: 5′-C/AGGAA/T-3′. This domain binds to DNA as a monomer, unlike many other DNA-binding proteins. The ETS domain of the PU.1 transcription factor has been crystallized in complex with a 16-base pair oligonucleotide that contains the recognition sequence. The crystals formed in the space group C2 with a = 89.1, b = 101.9, c = 55.6 Å, and β = 111.2° and diffract to at least 2.3 Å. There are two complexes in the asymmetric unit. Production of large usable crystals was dependent on the length of both protein and DNA components, the use of oligonucleotides with unpaired A and T bases at the termini, and the presence of polyethylene glycol and zinc acetate in the crystallization solutions. This is the first ETS domain to be crystallized, and the strategy used to crystallize this complex may be useful for other members of the ets family.
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影响因子：
56.9
作者：
HENDRICKSON, WA
通讯作者：
HENDRICKSON, WA
影响因子：
56.9
作者：
HARRISON, CJ;BOHM, AA;NELSON, HCM
通讯作者：
NELSON, HCM
影响因子：
2.9
作者：
DONALDSON, LW;PETERSEN, JM;MCINTOSH, LP
通讯作者：
MCINTOSH, LP
影响因子：
--
作者：
J. Puglisi;I. Tinoco
通讯作者：
J. Puglisi;I. Tinoco
用于带有多线区域检测器的衍射仪的软件。
影响因子：
--
作者：
Howard,AJ;Nielsen,C;Xuong,NH
通讯作者：
Xuong,NH