Gastrointestinal Surgery for Inflammatory Bowel Disease Persistently Lowers Microbiome and Metabolome Diversity.
Gastrointestinal Surgery for Inflammatory Bowel Disease Persistently Lowers Microbiome and Metabolome Diversity.
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DOI：
10.1093/ibd/izaa262
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发表时间：
2021-04-15
影响因子：
4.9
通讯作者：
Boland BS
中科院分区：
文献类型：
--
作者：
Fang X;Vázquez-Baeza Y;Elijah E;Vargas F;Ackermann G;Humphrey G;Lau R;Weldon KC;Sanders JG;Panitchpakdi M;Carpenter C;Jarmusch AK;Neill J;Miralles A;Dulai P;Singh S;Tsai M;Swafford AD;Smarr L;Boyle DL;Palsson BO;Chang JT;Dorrestein PC;Sandborn WJ;Knight R;Boland BS
许多研究已经调查了微生物组在炎症性肠病(IBD)中的作用，但很少有人专门关注手术或其对代谢组的影响，这些代谢组可能因手术类型而异，需要纵向采样。我们的目标是描述和对比IBD不同手术(包括回结肠切除和结肠切除)后微生物组和代谢组的变化。加州大学圣地亚哥分校IBD生物库前瞻性地收集了129名受试者(50名溃疡性结肠炎；79名克罗恩病)的332份粪便样本。在这些患者中，21名克罗恩病患者接受了回结肠切除，17名患者接受了结肠切除术。我们使用散弹枪元基因组学和非靶向液相色谱，然后串联质谱仪代谢组学来表征这些患者的微生物群和代谢体，直到最初采样后24个月。物种多样性和代谢物多样性在组间差异显著(物种多样性：Mann-Whitney U检验，P值 = 7.8E-17；代谢组学，P值 = 0.0043)。尤其是在接受手术的受试者中，大肠杆菌的相对丰度急剧增加。与代谢物图谱相比，物种图谱能够更好地根据手术状况对受试者进行分类(平均精确度为0.80比0.68)。肠道手术似乎降低了IBD患者肠道微生物组和代谢组的多样性，而且这些变化可能会持续下去。随着时间的推移，手术也会进一步破坏微生物组(但不是代谢组)的稳定性，即使是相对于先前确定的IBD患者微生物组的不稳定。这些长期影响及其对健康结果的后果需要在与微生物组相关的表型相关的前瞻性纵向试验中进行研究。
Many studies have investigated the role of the microbiome in inflammatory bowel disease (IBD), but few have focused on surgery specifically or its consequences on the metabolome that may differ by surgery type and require longitudinal sampling. Our objective was to characterize and contrast microbiome and metabolome changes after different surgeries for IBD, including ileocolonic resection and colectomy. The UC San Diego IBD Biobank was used to prospectively collect 332 stool samples from 129 subjects (50 ulcerative colitis; 79 Crohn’s disease). Of these, 21 with Crohn’s disease had ileocolonic resections, and 17 had colectomies. We used shotgun metagenomics and untargeted liquid chromatography followed by tandem mass spectrometry metabolomics to characterize the microbiomes and metabolomes of these patients up to 24 months after the initial sampling. The species diversity and metabolite diversity both differed significantly among groups (species diversity: Mann-Whitney U test P value = 7.8e-17; metabolomics, P-value = 0.0043). Escherichia coli in particular expanded dramatically in relative abundance in subjects undergoing surgery. The species profile was better able to classify subjects according to surgery status than the metabolite profile (average precision 0.80 vs 0.68). Intestinal surgeries seem to reduce the diversity of the gut microbiome and metabolome in IBD patients, and these changes may persist. Surgery also further destabilizes the microbiome (but not the metabolome) over time, even relative to the previously established instability in the microbiome of IBD patients. These long-term effects and their consequences for health outcomes need to be studied in prospective longitudinal trials linked to microbiome-involved phenotypes.
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影响因子：
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作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
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通讯作者：
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