Resolving structural isomers of monosaccharide methyl glycosides using drift tube and traveling wave ion mobility mass spectrometry.
Resolving structural isomers of monosaccharide methyl glycosides using drift tube and traveling wave ion mobility mass spectrometry.
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DOI：
10.1021/ac203116a
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发表时间：
2012-04-03
影响因子：
7.4
通讯作者：
Hill, Herbert H., Jr.
中科院分区：
文献类型：
--
作者：
Li, Hongli;Giles, Kevin;Bendiak, Brad;Kaplan, Kimberly;Siems, William F.;Hill, Herbert H., Jr.
单糖结构异构体，包括十六个甲基-D-吡喃葡萄糖苷和四个甲基-N-乙酰己糖胺进行离子迁移率的电喷雾离子迁移质谱测量。采用了两种离子迁移率-MS系统：大气压漂移管离子迁移率飞行时间质谱和Synapt G2 HDMS系统，该系统包含低压行波离子迁移率分离器。所有化合物均以[M+Na]+离子形式在正离子模式下进行研究。大多数的单糖结构异构体表现出不同的流动性漂移时间在任一系统中，这取决于它们的异头和立体化学构型的差异。一般而言，两种仪器之间的漂移时间模式（异构体的相对漂移时间）相匹配。使用大气压漂移管观察到更高的分辨率。根据大气压离子迁移率实验，直接计算单糖结构异构体的碰撞截面值，并制作了行波离子迁移率仪的碰撞截面校准曲线。总体而言，它被证明，离子迁移率质谱使用漂移管或行波离子迁移率是一种有价值的技术，用于解决单糖甲基糖苷的钠加合物之间的立体化学的细微变化。
Monosaccharide structural isomers including sixteen methyl-D-glycopyranosides and four methyl-N-acetylhexosamines were subjected to ion mobility measurements by electrospray ion mobility mass spectrometry. Two ion mobility-MS systems were employed: atmospheric pressure drift tube ion mobility time-of-flight mass spectrometry and a Synapt G2 HDMS system which incorporates a low pressure traveling wave ion mobility separator. All the compounds were investigated as [M+Na]+ ions in the positive mode. A majority of the monosaccharide structural isomers exhibited different mobility drift times in either system, depending on differences in their anomeric and stereochemical configurations. In general, drift time patterns (relative drift times of isomers) matched between the two instruments. Higher resolving power was observed using the atmospheric pressure drift tube. Collision cross section values of monosaccharide structural isomers were directly calculated from the atmospheric pressure ion mobility experiments and a collision cross section calibration curve was made for the traveling wave ion mobility instrument. Overall, it was demonstrated that ion mobility-mass spectrometry using either drift tube or traveling wave ion mobility is a valuable technique for resolving subtle variations in stereochemistry among the sodium adducts of monosaccharide methyl glycosides.
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影响因子：
2.2
作者：
Armstrong, GS;Mandelshtam, VA;Bendiak, B
通讯作者：
Bendiak, B
影响因子：
2.7
作者：
Jones, DNM;Bendiak, B
通讯作者：
Bendiak, B
影响因子：
1.8
作者：
Cerda, BA;Wesdemiotis, C
通讯作者：
Wesdemiotis, C
影响因子：
3.3
作者：
Fenn, Larissa S.;McLean, John A.
通讯作者：
McLean, John A.
影响因子：
2
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
通讯作者：
Campuzano, Iain