Effect of taurine in combination with electroacupuncture on neuronal damage following transient focal cerebral ischemia in rats.
Effect of taurine in combination with electroacupuncture on neuronal damage following transient focal cerebral ischemia in rats.
复制标题
牛磺酸联合电针对大鼠短暂性局灶性脑缺血后神经元损伤的影响。
DOI：
10.3727/036012902816026086
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发表时间：
2002
影响因子：
0.3
通讯作者：
程介士
中科院分区：
文献类型：
--
作者：
郭景春;李荣;赵鹏;程介士
本研究旨在探讨牛磺酸是否有利于电针（EA）对短暂性大脑中动脉闭塞（MCAO）所致大鼠脑缺血的神经保护作用。采用H&E染色和TUNEL（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记）法检测组织学变化和DNA损伤程度。牛磺酸和电针联合组可以显着降低纹状体缺血性损伤引起的梗死面积和细胞损失的百分比（p<0.05，与单独使用牛磺酸或电针治疗相比），并且TUNEL阳性细胞的百分比也小于两个单独治疗组（p<0.05）。此外，Bcl-2（促进细胞存活）、Bax（促进细胞死亡）和P53（抑癌基因p53的产物）的免疫反应性检测显示，联合组明显减弱了P53免疫反应性表达，而增强了皮质中Bcl-2与Bax免疫反应性表达比。这些结果提示，牛磺酸与电针联合使用可以发挥更好的神经保护作用，其可能与调节细胞凋亡通路有关。
The present study was to investigate whether taurine is beneficial to the neuroprotective effects of electroacupuncture (EA) on rat cerebral ischemia induced by transient Middle Cerebral Artery Occlusion (MCAO). Histological change and DNA damage degree were detected by H&E staining and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method. Taurine and EA combination group could significantly decrease the percentages of infarct area and cell loss induced by ischemic injury in the striatum (p<0.05, vs singly treated with taurine or EA), and also the percentage of TUNEL-positive cells was smaller than both singly treated groups (p<0.05). Moreover, immunoreactivities detection of Bcl-2 (which promotes cell survival), Bax (which promotes cell death) and P53 (product of tumor suppressor gene p53) showed that the combination group apparently attenuated P53 immunoreactivities expression whereas augmented the immunoreactivities expression ratio of Bcl-2 to Bax in cortex. These results suggested that combining use of taurine and EA could exert better neuroprotective effects, which might be related to the regulation of apoptosis pathway.
DOI：
10.1523/jneurosci.17-09-03064.1997
发表时间：
1997-05
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
Z. Pang;J. Geddes
通讯作者：
Z. Pang;J. Geddes
影响因子：
6.1
作者：
SCHURR, A;TSENG, MT;RIGOR, BM
通讯作者：
RIGOR, BM
影响因子：
4.7
作者：
Muriel B. O'byrne;K. Tipton
通讯作者：
Muriel B. O'byrne;K. Tipton