Two organobromines trigger lifespan, growth, reproductive and transcriptional changes in Caenorhabditis elegans
Two organobromines trigger lifespan, growth, reproductive and transcriptional changes in Caenorhabditis elegans
复制标题
两种有机溴会引发秀丽隐杆线虫的寿命、生长、繁殖和转录变化
DOI：
10.1007/s11356-014-2932-6
复制
发表时间：
2014
影响因子：
5.8
通讯作者：
Steinberg CEW
中科院分区：
文献类型：
--
作者：
Saul N;Baberschke N;Chakrabarti S;Stürzenbaum SR;Lieke T;Menzel R;Jonáš A;Steinberg CEW
天然和人工来源的有机溴在水生和陆生环境中无处不在。虽然已经确定暴露于高浓度有机溴对脊椎动物有害，但很少有研究调查环境实际浓度对无脊椎动物的影响。本研究采用两种有机溴，即二溴乙酸（DBAA）和四溴双酚a (TBBP)，对秀丽隐杆线虫（decaenorhabditis elegans）进行了攻击，并监测了不同生命特征变量和整体基因表达模式的变化。50微摩尔DBAA刺激线虫的生长和寿命；然而，繁殖的开始被推迟了。相比之下，TBBP以一种致效方式改变了寿命，即在0.1 μM时受到刺激，但在50 μM时受损。在2 μM TBBP下，繁殖性能甚至受到损害。此外，DBAA不能降低TBBP的毒性作用。全基因组DNA微阵列显示，这两种有机溴都削弱了信号传导和神经过程。此外，在转录水平上，50 μM TBBP诱导蛋白水解，DBAA上调生物合成和代谢。综上所述，即使是自然存在的有机溴浓度也会影响秀丽隐杆线虫的生物分子反应和生命周期特征。寿命的延长伴随着生殖行为的负面变化，这对人口的稳定至关重要。因此，本文强调暴露于适度的、符合环境实际浓度的有机溴的影响不应被忽视。
Organobromines of natural and artificial origin are omnipresent in aquatic and terrestrial environments. Although it is well established that exposure to high concentrations of organobromines are harmful to vertebrates, few studies have investigated the effect of environmentally realistic concentrations on invertebrates. Here, the nematodeCaenorhabditis eleganswas challenged with two organobromines, namely dibromoacetic acid (DBAA) and tetrabromobisphenol-A (TBBP), and monitored for changes in different life trait variables and global gene expression patterns. Fifty micromolar DBAA stimulated the growth and lifespan of the nematodes; however, the onset of reproduction was delayed. In contrast, TBBP changed the lifespan in a hormetic fashion, namely it was stimulated at 0.1 μM but impaired at 50 μM. The reproductive performance was even impaired at 2 μM TBBP. Moreover, DBAA could not reduce the toxic effect of TBBP when applied as a mixture. A whole-genome DNA microarray revealed that both organobromines curtailed signalling and neurological processes. Furthermore on the transcription level, 50 μM TBBP induced proteolysis and DBAA up-regulated biosynthesis and metabolism. To conclude, even naturally occurring concentrations of organobromines can influence the biomolecular responses and life cycle traits inC. elegans. The life extension is accompanied by negative changes in the reproductive behaviour, which is crucial for the stability of populations. Thus, this paper highlights that the effects of exposure to moderate, environmentally realistic concentrations of organobromines should not be ignored.
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影响因子：
4
作者：
James, Catherine E.;Davey, Mary W.
通讯作者：
Davey, Mary W.
通过秀丽隐杆线虫测试对两种有机溴模型化合物和天然含 AOBr 地表水样进行神经毒性评估
影响因子：
6.8
作者：
Ju, Jingjuan;Lieke, Thora;Steinberg, Christian E. W.
通讯作者：
Steinberg, Christian E. W.
上层结构中的教育组织和超滤研究
DOI：
--
发表时间：
2007
期刊：
影响因子：
--
作者：
A. Hütteroth
通讯作者：
A. Hütteroth
地表水中溴化有机化合物的出现和来源。
影响因子：
8.8
作者：
A. Putschew;Mirko Mania;M. Jekel
通讯作者：
M. Jekel
影响因子：
2.6
作者：
P. Manninen;E. Häsänen
通讯作者：
E. Häsänen