Binding to sialic acids is not an essential step for the entry of animal rotaviruses to epithelial cells in culture
Binding to sialic acids is not an essential step for the entry of animal rotaviruses to epithelial cells in culture
复制标题
与唾液酸的结合并不是动物轮状病毒进入培养物上皮细胞的必要步骤
作者：
E. Méndez;C. Arias;S. López
动物轮状病毒对靶细胞的感染需要细胞表面存在唾液酸。用神经氨酸酶处理细胞或用一些唾液糖蛋白（如糖蛋白A）孵育病毒，可大大减少病毒的结合，从而降低病毒的传染性。在这项工作中，我们报道了分离的动物轮状病毒变种，其传染性不再依赖于细胞表面唾液酸的存在。此外，尽管这些变体与糖蛋白A结合的效率与野生型病毒一样高，但这种相互作用不再抑制病毒的传染性。这些观察结果表明，突变体与细胞的初始相互作用发生在与位于VP8上的唾液酸结合位点不同的位置，VP8是VP4较小的胰蛋白酶裂解产物。重组分析显示突变型与VP4基因分离。对针对VP4和VP7的中和单克隆抗体进行了测试，以检测其中和变异的能力。VP4的胰蛋白酶裂解产物VP7和VP5抗体能像野生型病毒一样有效地中和突变体。相比之下，尽管VP8抗体能够与突变体结合，但它们表现出很少或没有中和活性。这些发现的意义在轮状病毒附着和渗透上皮细胞的培养进行了讨论。
The infection of target cells by animal rotaviruses requires the presence of sialic acids on the cell surface. Treatment of the cells with neuraminidases or incubation of the viruses with some sialoglycoproteins, such as glycophorin A, greatly reduces virus binding, with the consequent reduction of viral infectivity. In this work, we report the isolation of animal rotavirus variants whose infectivity is no longer dependent on the presence of sialic acids on the cell surface. In addition, although these variants bind to glycophorin A as efficiently as the wild-type virus, this interaction no longer inhibit viral infectivity. These observations indicate that the initial interaction of the mutants with the cell occurs at a site different from the sialic acid-binding site located on VP8, the smaller trypsin cleavage product of VP4. Reassortant analysis showed that the mutant phenotype segregates with the VP4 gene. Neutralizing monoclonal antibodies directed to VP4 and VP7 were tested for their ability to neutralize the variants. Antibodies to VP7 and VP5, the larger trypsin cleavage product of VP4, neutralized the mutants as efficiently as the wild-type virus. In contrast, although antibodies to VP8 were able to bind to the mutants, they showed little or no neutralizing activity. The implications of these findings in rotavirus attachment to and penetration of epithelial cells in culture are discussed.
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轮状病毒的细胞表面配体：小鼠肠道糖脂和合成碳水化合物类似物。
影响因子：
3.7
作者：
Srnka,CA;Tiemeyer,M;Gilbert,JH;Moreland,M;Schweingruber,H;deLappe,BW;James,PG;Gant,T;Willoughby,RE;Yolken,RH
通讯作者：
Yolken,RH
影响因子：
3.7
作者：
FIORE, L;GREENBERG, HB;MACKOW, ER
通讯作者：
MACKOW, ER
DOI：
10.1172/jci112775
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
R. Yolken;R. Willoughby;Siok-Bi Wee;R. Miskuff;S. Vonderfecht
通讯作者：
R. Yolken;R. Willoughby;Siok-Bi Wee;R. Miskuff;S. Vonderfecht
影响因子：
5.6
作者：
PRASAD, BVV;WANG, GJ;CHIU, W
通讯作者：
CHIU, W