Cloning of Three Cytokinin Oxidase/Dehydrogenase Genes in Bambusa oldhamii.
Cloning of Three Cytokinin Oxidase/Dehydrogenase Genes in Bambusa oldhamii.
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DOI：
10.3390/cimb45030123
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发表时间：
2023-02-27
影响因子：
3.1
通讯作者：
Hsieh, Lu-Sheng
中科院分区：
文献类型：
--
作者：
Hsieh, Chun-Yen;Hsieh, Lu-Sheng
细胞分裂素氧化酶/脱氢酶(CKX)催化细胞分裂素的不可逆分解，细胞分裂素是一类调节细胞分裂的植物激素。根据单子叶植物CKX基因的保守序列，设计了用于竹子基因组文库筛选的探针。3个细胞分裂素氧化酶基因的克隆结果如下：BoCKX1、BoCKX2和BoCKX3。比较三个基因的外显子-内含子结构，BoCKX1和BoCKX3基因有三个外显子和两个内含子，而BoCKX2基因包含四个外显子和三个内含子。BoCKX2蛋白的氨基酸序列与BoCKX1和BoCKX3蛋白的同源性分别为78%和79%。BoCKX1和BoCKX3基因的氨基酸和核苷酸序列同源性都在90%以上，因此它们的亲缘关系特别密切。这三种BoCKX蛋白都携带典型的分泌途径的信号肽序列，并且在N端的黄素腺嘌呤二核苷酸(FAD)结合区发现了GHS基序，这表明BoCKX蛋白可能通过预测的组氨酸残基与FAD辅因子共价结合。
Cytokinin oxidase/dehydrogenase (CKX) catalyzes the irreversible breakdown of active cytokinins, which are a class of plant hormones that regulate cell division. According to conserved sequences of CKX genes from monocotyledons, PCR primers were designed to synthesize a probe for screening a bamboo genomic library. Cloned results of three genes encoding cytokinin oxidase were named as follows: BoCKX1, BoCKX2, and BoCKX3. In comparing the exon-intron structures among the above three genes, there are three exons and two introns in BoCKX1 and BoCKX3 genes, whereas BoCKX2 contains four exons and three introns. The amino acid sequence of BoCKX2 protein shares 78% and 79% identity with BoCKX1 and BoCKX3 proteins, respectively. BoCKX1 and BoCKX3 genes are particularly closely related given that the amino acid and nucleotide sequence identities are more than 90%. These three BoCKX proteins carried putative signal peptide sequences typical of secretion pathway, and a GHS-motif was found at N-terminal flavin adenine dinucleotide (FAD) binding domain, suggesting that BoCKX proteins might covalently conjugate with an FAD cofactor through a predicted histidine residue.
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