Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement.
Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement.
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DOI：
10.1111/pbi.13595
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发表时间：
2021-05
影响因子：
13.8
通讯作者：
Jameson PE
中科院分区：
文献类型：
--
作者：
Chen L;Zhao J;Song J;Jameson PE
细胞分裂素是N6-取代的腺嘌呤衍生物，控制着作物生产力的关键方面。细胞分裂素水平通过异戊烯基转移酶（IPT）的生物合成、细胞分裂素氧化酶/脱氢酶（CKX）的破坏和细胞分裂素葡糖基转移酶（CGT）通过葡糖基化的失活来控制。虽然产量构成因素和对干旱和相关非生物胁迫的耐受性已经通过操纵IPT和/或CKX表达得到了积极的解决，但对CGT的关注要少得多。由于CGT的命名尚不清楚，我们建议用COGT、CNGT、CONGT和hocGT来描述O-、N-和双功能CGT。由于水稻和拟南芥的特定CGT突变体都显示出对产量构成因素的影响，我们查询了小麦基因组数据库IWGSC RefSeq v1.0和v2.0，以研究小麦CGT。除了为53种小麦CGT提供明确的名称外，我们还通过审查1000多个RNA-seq数据集，展示了它们在70种发育组织中的表达模式及其对各种胁迫条件的反应特征。这些揭示了各种反应模式，并表明生殖组织中的表达通常比营养组织中的表达更有限。多个顺式调节元件存在于53个CGT的起始密码子上游3 kb处。与脱落酸，光和茉莉酸甲酯相关的元素特别多，表明CGTs对环境的反应性。这些数据集表明CGT对小麦改良具有潜在价值，并且这些可以在TILLING或基因编辑小麦育种计划中作为目标。
The cytokinins, which are N6‐substituted adenine derivatives, control key aspects of crop productivity. Cytokinin levels are controlled via biosynthesis by isopentenyl transferase (IPT), destruction by cytokinin oxidase/dehydrogenase (CKX), and inactivation via glucosylation by cytokinin glucosyl transferases (CGTs). While both yield components and tolerance to drought and related abiotic stressors have been positively addressed via manipulation of IPT and/or CKX expression, much less attention has been paid to the CGTs. As naming of the CGTs has been unclear, we suggest COGT, CNGT, CONGT and CNOGT to describe the O‐, N‐ and dual function CGTs. As specific CGT mutants of both rice and arabidopsis showed impacts on yield components, we interrogated the wheat genome database, IWGSC RefSeq v1.0 & v2.0, to investigate wheat CGTs. Besides providing unambiguous names for the 53 wheat CGTs, we show their expression patterns in 70 developmental tissues and their response characteristics to various stress conditions by reviewing more than 1000 RNA‐seq data sets. These revealed various patterns of responses and showed expression generally being more limited in reproductive tissues than in vegetative tissues. Multiple cis‐regulatory elements are present in the 3 kb upstream of the start codons of the 53 CGTs. Elements associated with abscisic acid, light and methyl jasmonate are particularly over‐represented, indicative of the responsiveness of CGTs to the environment. These data sets indicate that CGTs have potential value for wheat improvement and that these could be targeted in TILLING or gene editing wheat breeding programmes.
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