Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.
Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.
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DOI：
10.1016/j.virol.2009.01.013
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发表时间：
2009-04-25
期刊：
影响因子：
3.7
通讯作者：
Wong JK
中科院分区：
文献类型：
--
作者：
Yukl S;Pillai S;Li P;Chang K;Pasutti W;Ahlgren C;Havlir D;Strain M;Günthard H;Richman D;Rice AP;Daar E;Little S;Wong JK
艾滋病毒在CD4+淋巴细胞中建立潜伏感染的能力是根除艾滋病毒的主要障碍。目前尚不清楚是什么机制有利于潜伏感染而不是生产性感染，但之前的研究表明，病毒转录因子Tat或其RNA靶标TAR可能起到了作用。使用在感染后6个月内开始接受抗逆转录病毒治疗并达到病毒载量50(抑制)的五个人的样本，我们从基线血浆和在基线和抑制时间点获得的CD4+T细胞的共培养中分离出体外繁殖的HIV病毒的单外显子和双外显子TAT RNA。与来自基线血浆的病毒(主要来自生产性感染的CD4+T细胞)相比，来自基线和抑制共培养的病毒(主要来自潜伏感染的细胞)具有更多反式激活活性受损的TAT变体。这些发现表明，TAT-TAR轴的活性受损可能有助于在CD4+T细胞中建立潜伏感染。
The ability of HIV to establish latent infection in CD4+ lymphocytes represents a major barrier to the eradication of HIV. It is not clear what mechanisms favor latent over productive infection, but prior studies have suggested a role for the viral transcription factor Tat or its RNA target, TAR. Using samples from five individuals who were started on ART within 6 months of infection and achieved a viral load <50 (suppressed), we isolated one- and two-exon tat RNA from HIV propagated ex vivo from baseline plasma and from co-cultures of CD4+ T cells obtained at baseline and suppressed time points. Compared to virus from the baseline plasma (mostly from productively-infected CD4+ T cells), virus from the baseline and suppressed co-cultures (mostly from latently-infected cells) had more Tat variants with impaired transactivation activity. These findings suggest that impaired activity in the Tat-TAR axis may contribute to the establishment of latent infection in CD4+ T cells.
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影响因子：
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作者：
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