Seawater acclimation causes independent alterations in Na+/K+- and H+-ATPase activity in isolated mitochondria-rich cell subtypes of the rainbow trout gill
Seawater acclimation causes independent alterations in Na+/K+- and H+-ATPase activity in isolated mitochondria-rich cell subtypes of the rainbow trout gill
复制标题
海水驯化导致虹鳟鱼鳃分离的富含线粒体的细胞亚型中 Na /K - 和 H - ATP 酶活性发生独立变化
DOI：
10.1242/jeb.00844
复制
发表时间：
2004
影响因子：
2.8
通讯作者：
G. Goss
中科院分区：
文献类型：
--
作者：
G. Hawkings;F. Galvez;G. Goss
摘要虹鳟鱼鳃中富含线粒体的细胞（MR细胞）在适应部分强度（10‰）和全强度（30‰）海水的过程中会发生相对分布和生化功能的变化。当鳟鱼适应10%或30%海水时，分离的总鳃细胞中Na+/K+-ATP酶活性增加五倍，H+-ATP酶活性降低四倍。当根据与花生凝集素凝集素 (PNA) 的差异结合来分离总 MR 鳃细胞时，PNA 亚型在适应海水的鱼中的相对分布发生了变化。在淡水中，PNA–:PNA+ 的比例为 65:35，而在海水中，PNA–:PNA+ 的分布变为 20:80。此外，在海水适应过程中，每种独立细胞类型的 Na+/K+-ATP 酶和 H+-ATP 酶活性发生了差异变化。 PNA– 细胞中的 Na+/K+-ATP 酶活性增加了 197%，而 PNA+ 细胞中的 Na+/K+-ATP 酶活性降低了 57%。然而，在适应海水期间，PNA– (84%) 和 PNA+ (72%) 亚型的 H+-ATPase 活性均下降。
SUMMARY Mitochondria-rich cells (MR cells) of the gills of rainbow trout undergo changes in relative distribution and biochemical function during acclimation to partial-strength (10‰) and full-strength (30‰) seawater. In isolated total gill cells, Na+/K+-ATPase activity increased fivefold and H+-ATPase activity decreased fourfold when trout were acclimated to either 10‰ or 30‰ seawater. When total MR gill cells were separated based on differential binding to peanut lectin agglutinin (PNA), the PNA subtypes underwent a change in relative distribution in seawater-acclimated fish. In freshwater, the ratio of PNA–:PNA+ was 65:35 while in seawater the distribution changed to 20:80 PNA–:PNA+. Additionally, differential changes in Na+/K+-ATPase and H+-ATPase activity in each of the independent cell types occurred during seawater acclimation; Na+/K+-ATPase activity in the PNA– cells increased by 197% while in PNA+ cells Na+/K+-ATPase decreased by 57%. However, H+-ATPase activity was decreased in both PNA– (84%) and PNA+ (72%) subtypes during acclimation to seawater.
DOI：
--
发表时间：
1999-02
期刊：
The Journal of experimental biology
影响因子：
--
作者：
J. B. Claiborne;C. Blackston;Keith P. Choe;D. C. Dawson;S. Harris;L. Mackenzie;A. Morrison-Shetlar
通讯作者：
J. B. Claiborne;C. Blackston;Keith P. Choe;D. C. Dawson;S. Harris;L. Mackenzie;A. Morrison-Shetlar
DOI：
--
发表时间：
2000
期刊：
The Journal of experimental biology
影响因子：
--
作者：
D. Brown;S. Breton
通讯作者：
D. Brown;S. Breton
影响因子：
--
作者：
SATLIN, LM;MATSUMOTO, T;SCHWARTZ, GJ
通讯作者：
SCHWARTZ, GJ