Oral mucosa stem cells alleviates spinal cord injury-induced neurogenic bladder symptoms in rats.
Oral mucosa stem cells alleviates spinal cord injury-induced neurogenic bladder symptoms in rats.
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DOI：
10.1186/1423-0127-21-43
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发表时间：
2014-05-13
影响因子：
11
通讯作者：
Kim KH
中科院分区：
文献类型：
--
作者：
Cho YS;Ko IG;Kim SE;Lee SM;Shin MS;Kim CJ;Kim SH;Jin JJ;Kim KH
脊髓损伤(SCI)会导致各种生理功能的恶化，尤其是由于脊髓损伤而导致的膀胱问题。干细胞治疗脊髓损伤是治疗脊髓损伤、恢复功能的新策略。口腔黏膜细胞被认为是干细胞样祖细胞。在本研究中，我们研究了口腔粘膜干细胞对脊髓损伤所致神经源性膀胱的作用及其与神经细胞凋亡和细胞增殖的关系。大鼠脊髓损伤后，膀胱收缩压力和收缩时间增加，而口腔黏膜干细胞移植可降低脊髓损伤大鼠的收缩压力和收缩时间。脊髓损伤诱导脊髓组织细胞凋亡，而口腔粘膜干细胞治疗抑制脊髓损伤诱导的细胞凋亡。口腔黏膜干细胞移植可改善脊髓损伤后的脊髓损伤，损伤组织周围有新的组织生长。此外，口腔粘膜干细胞移植抑制了脊髓损伤诱导的排尿中心神经元的激活。口腔黏膜干细胞移植可通过抑制细胞凋亡和促进细胞增殖来改善脊髓损伤后神经源性膀胱症状。结果表明，脊髓损伤后大鼠排尿中枢神经元的激活受到抑制，排尿功能恢复正常。
Spinal cord injury (SCI) deteriorates various physical functions, in particular, bladder problems occur as a result of damage to the spinal cord. Stem cell therapy for SCI has been focused as the new strategy to treat the injuries and to restore the lost functions. The oral mucosa cells are considered as the stem cells-like progenitor cells. In the present study, we investigated the effects of oral mucosa stem cells on the SCI-induced neurogenic bladder in relation with apoptotic neuronal cell death and cell proliferation. The contraction pressure and the contraction time in the urinary bladder were increased after induction of SCI, in contrast, transplantation of the oral mucosa stem cells decreased the contraction pressure and the contraction time in the SCI-induced rats. Induction of SCI initiated apoptosis in the spinal cord tissues, whereas treatment with the oral mucosa stem cells suppressed the SCI-induced apoptosis. Disrupted spinal cord by SCI was improved by transplantation of the oral mucosa stem cells, and new tissues were increased around the damaged tissues. In addition, transplantation of the oral mucosa stem cells suppressed SCI-induced neuronal activation in the voiding centers. Transplantation of oral mucosa stem cells ameliorates the SCI-induced neurogenic bladder symptoms by inhibiting apoptosis and by enhancing cell proliferation. As the results, SCI-induced neuronal activation in the neuronal voiding centers was suppressed, showing the normalization of voiding function.
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