The Regulation of Chemerin and CMKLR1 Genes Expression by TNF-α, Adiponectin, and Chemerin Analog in Bovine Differentiated Adipocytes.
The Regulation of Chemerin and CMKLR1 Genes Expression by TNF-α, Adiponectin, and Chemerin Analog in Bovine Differentiated Adipocytes.
复制标题
DOI：
10.5713/ajas.2012.12083
复制
发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Roh SG
中科院分区：
文献类型：
--
作者：
Suzuki Y;Hong YH;Song SH;Ardiyanti A;Kato D;So KH;Katoh K;Roh SG
脂肪因子是脂肪细胞衍生的蛋白质，在包括能量稳态在内的各种生理学中具有重要作用。 Chemerin 是脂肪细胞衍生的脂肪因子之一，在分化的脂肪细胞中高表达，已知可诱导巨噬细胞趋化性和葡萄糖不耐受。本研究的目的是研究牛脂肪细胞分化过程中以及用肿瘤坏死因子-α (TNF-α)、脂联素、瘦素和凯莫瑞（肽类似物）处理的分化脂肪细胞中凯莫瑞和趋化因子样受体 1 (CMKLR1) 基因表达水平的变化。凯莫瑞基因的表达水平在分化期第6天和第12天增加，伴随着细胞质脂滴的增加。从第6天开始，过氧化物酶体增殖物激活受体-γ2（PPAR-γ2）基因表达水平显着高于第0天和第3天。相反，CMKLR1表达水平在分化期结束时下降。在完全分化的脂肪细胞中（即第 12 天），TNF-α 和脂联素的治疗上调了凯莫瑞和 CMKLR1 基因表达水平，尽管瘦素没有表现出这种效果。此外，无论剂量如何，凯莫瑞类似物治疗均显示上调凯莫瑞基因表达水平。这些结果表明，牛脂肪细胞中凯莫瑞的表达可能受到凯莫瑞本身和其他脂肪因子的调节，这表明其可能在调节脂肪组织中脂肪因子的分泌中发挥作用。
Adipokines, adipocyte-derived protein, have important roles in various kinds of physiology including energy homeostasis. Chemerin, one of adipocyte-derived adipokines, is highly expressed in differentiated adipocytes and is known to induce macrophage chemotaxis and glucose intolerance. The objective of the present study was to investigate the changes of chemerin and the chemokine-like-receptor 1 (CMKLR1) gene expression levels during differentiation of the bovine adipocyte and in differentiated adipocytes treated with tumor necrosis factor-α (TNF-α), adiponectin, leptin, and chemerin (peptide analog). The expression levels of the chemerin gene increased at d 6 and 12 of the differentiation period accompanied by increased cytoplasm lipid droplets. From d 6 onward, peroxisome proliferator-activated receptor-γ2 (PPAR-γ2) gene expression levels were significantly higher than that of d 0 and 3. In contrast, CMKLR1 expression levels decreased at the end of the differentiation period. In fully differentiated adipocytes (i.e. at d 12), the treatment of TNF-α and adiponectin upregulated both chemerin and CMKLR1 gene expression levels, although leptin did not show such effects. Moreover, chemerin analog treatment was shown to upregulate chemerin gene expression levels regardless of doses. These results suggest that the expression of chemerin in bovine adipocyte might be regulated by chemerin itself and other adipokines, which indicates its possible role in modulating the adipokine secretions in adipose tissues.
登录
查看更多内容
影响因子：
4.8
作者：
Muruganandan, Shanmugam;Parlee, Sebastian D.;Sinal, Christopher J.
通讯作者：
Sinal, Christopher J.
影响因子：
82.9
作者：
Yamauchi, T;Kamon, J;Kadowaki, T
通讯作者：
Kadowaki, T
影响因子：
4.8
作者：
Combs, TP;Pajvani, UB;Scherer, PE
通讯作者：
Scherer, PE
影响因子：
4.8
作者：
Bozaoglu, Kiymet;Bolton, Kristy;Segal, David
通讯作者：
Segal, David
DOI：
10.1016/j.bbrc.2007.08.104
发表时间：
2007-11-03
影响因子：
3.1
作者：
Roh, Sang-Gun;Song, Sang-Houn;Sasaki, Shin-ichi
通讯作者：
Sasaki, Shin-ichi