RacA and the Soj‐Spo0J system combine to effect polar chromosome segregation in sporulating Bacillus subtilis
RacA and the Soj‐Spo0J system combine to effect polar chromosome segregation in sporulating Bacillus subtilis
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RacA 和 Soj-Spo0J 系统结合影响枯草芽孢杆菌孢子中的极性染色体分离
作者：
L. Wu;J. Errington
枯草芽孢杆菌的孢子细胞经历高度极化的细胞分裂，并具有特殊的机制，可在分隔前将染色体的 oriC 区域移动到细胞极附近。定位于细胞极的 DivIVA 蛋白以及与染色体相关的 Soj 和 Spo0J 蛋白是将染色体传递到细胞极的机制的一部分。孢子形成特异性蛋白 RacA 编码第三种 DNA 结合蛋白，该蛋白与 Soj 和 Spo0J 协同作用，实现有效的极性染色体分离。 divIVA 突变体和 soj racA 双突变体具有意想不到的表型，其中 oriC 左侧和右侧的特定标记可以在前孢子室中捕获，但中央 oriC 区域被有效排除。这种“残留”捕获需要 Spo0J 蛋白。我们建议 Soj RacA DivIVA 系统需要从营养染色体分离机制确定的位置提取 oriC 区域，并将其锚定到细胞极。
Sporulating cells of Bacillus subtilis undergo a highly polarized cell division and possess a specialized mechanism to move the oriC region of the chromosome close to the cell pole before septation. DivIVA protein, which localizes to the cell pole, and the Soj and Spo0J proteins, which associate with the chromosome, are part of the mechanism that delivers the chromosome to the cell pole. A sporulation‐specific protein, RacA, encodes a third DNA‐binding protein, which acts in conjunction with Soj and Spo0J to effect efficient polar chromosome segregation. divIVA mutants and soj racA double mutants have an unexpected phenotype in which specific markers to the left and right of oriC can be captured in the prespore compartment but the central oriC region is efficiently excluded. This ‘residual’ trapping requires Spo0J protein. We suggest that the Soj RacA DivIVA system is required to extract the oriC region from its position determined by the vegetative chromosome segregation machinery and anchor it to the cell pole.
影响因子：
16
作者：
Quisel, JD;Lin, DCH;Grossman, AD
通讯作者：
Grossman, AD