Integrated Analysis of Molybdenum Nutrition and Nitrate Metabolism in Strawberry
Integrated Analysis of Molybdenum Nutrition and Nitrate Metabolism in Strawberry
复制标题
草莓钼营养与硝酸盐代谢的综合分析
DOI：
10.3389/fpls.2020.01117
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发表时间：
2020-07
影响因子：
5.6
通讯作者：
Fengshan Ren
中科院分区：
文献类型：
--
作者：
Li Liu;Hongmei Shi;Shaoxuan Li;Mingyue Sun;Rui Zhang;Yongmei Wang;Fengshan Ren
钼（Mo）是硝酸还原酶（NR）的Mo辅因子（Moco）的组分，因此对硝酸盐代谢至关重要。and.is然而，有关草莓缺钼的表型以及钼吸收和转运的生理和分子机制如何影响硝酸盐的吸收和利用，目前还知之甚少。本研究利用生理学和细胞学技术对草莓缺钼表型进行了鉴定。MoO_（4.2-）培养的幼苗生长良好，叶片和根的显微和超微结构正常。而缺钼条件下的幼苗则表现出叶片黄化、叶绿体不规则、膜结构不清晰等类似于氮素缺乏的超微结构变化。我们克隆并分析了一个推测的β-内酰胺转运蛋白FaMOT 1，它可能编码一个参与β-内酰胺摄取和转运的β-内酰胺转运蛋白。有趣的是，添加钼类似物钨酸盐导致较低的组织钼浓度，减少了钼从根到芽的转运，并增加了植物对钼缺乏的敏感性。MoO4.2培养的幼苗改变了Moco生物合成基因的表达。如预期的，NR活性在足够的MoO4.2-水平下更高。此外，缺钼胁迫下，15NO_3 ~-的运转和利用效率均降低。这些研究结果代表了重要的一步。了解如何运输，浓度和缺乏症状。影响草莓硝酸盐的吸收和利用。
Molybdenum (Mo) is a component of the Mo cofactor (Moco) of nitrate reductase (NR) and.is therefore essential for nitrate metabolism. However, little is known about Mo deficiency.phenotypes or about how physiological and molecular mechanisms of Mo uptake and.transport influence nitrate uptake and utilization in strawberry. Here, we used physiological.and cytological techniques to identify Mo deficiency phenotypes in strawberry. Seedlings.cultured with MoO4.2- grew well and exhibited normal microstructure and ultrastructure of.leaves and roots. By contrast, seedlings cultivated under Mo-deficient conditions showed.yellow leaf blades and ultrastructural changes such as irregular chloroplasts and unclear.membrane structures that were similar to the symptoms of nitrogen deficiency. We cloned.and analyzed a putative molybdate transporter, FaMOT1, which may encode a molybdate.transporter involved inthe uptake andtranslocation of molybdate. Interestingly,the addition of.the molybdate analog tungstate led to lower tissue Mo concentrations, reduced the.translocation of Mo from roots to shoots, and increased the plants’ sensitivity to Mo.deficiency. Seedlings cultivated with MoO4.2- altered expression of genes in Moco.biosynthesis. As expected, NR activity was higher under sufficient MoO4.2- levels..Furthermore, seedlings grown on Mo-deficient medium exhibited decreased 15NO3-.translocation and lower 15NO3- use efficiency. These findings represent an important step.towards understanding how molybdate transport, concentration, and deficiency symptoms.influence nitrate uptake and utilization in strawberry.
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影响因子：
3
作者：
Wendl MC
通讯作者：
Wendl MC
影响因子：
4.9
作者：
VONUEXKULL, HR;MUTERT, E
通讯作者：
MUTERT, E
影响因子：
3
作者：
Mendel, Ralf R.;Leimkuehler, Silke
通讯作者：
Leimkuehler, Silke
DOI：
10.1016/j.jtemb.2014.10.008
发表时间：
2015-01-01
影响因子：
3.5
作者：
Filipiak-Szok, Anna;Kurzawa, Marzanna;Szlyk, Edward
通讯作者：
Szlyk, Edward
影响因子：
3.6
作者：
M. Zahalak;Brenda S. Pratte;Kelly Werth;T. Thiel
通讯作者：
M. Zahalak;Brenda S. Pratte;Kelly Werth;T. Thiel