Listerin-dependent nascent protein ubiquitination relies on ribosome subunit dissociation.
Listerin-dependent nascent protein ubiquitination relies on ribosome subunit dissociation.
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DOI：
10.1016/j.molcel.2013.04.015
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发表时间：
2013-06-06
期刊：
影响因子：
16
通讯作者：
Hegde, Ramanujan S.
中科院分区：
文献类型：
--
作者：
Shao, Sichen;von der Malsburg, Karina;Hegde, Ramanujan S.
缺陷mRNA的质量控制依赖于它们的翻译来检测病变。因此，异常蛋白质是mRNA监视的专性副产物，必须被降解以避免破坏蛋白质稳态。这些有缺陷的翻译产物被认为是在核糖体泛素化，但泛素连接酶对这些核糖体的选择性机制尚不清楚。在这里，我们在体外重建由异常mRNA产生的新生蛋白质的泛素化。停滞的80 S核糖体-新生链复合物被核糖体再循环因子Hbs 1/Pelota/ABCE 1解离成独特的60 S-新生链-tRNA复合物。泛素连接酶Listerin优先识别60 S-新生链并触发有效的新生链泛素化。干扰Hbs 1功能稳定80 S复合物，排除有效的李斯特蛋白募集，并减少新生链泛素化。因此，核糖体再循环因子控制Listerin定位，解释了mRNA监视的翻译产物如何有效地泛素化，同时保留翻译核糖体。停滞核糖体上的新生蛋白泛素化已在体外重建分离的60 S亚基上的泛素化强烈优于完整的80 S核糖体上的泛素化60 S亚基内的新生链提示泛素连接酶的稳定结合李斯特蛋白通过核糖体再循环因子的亚基解离使连接酶募集
Quality control of defective mRNAs relies on their translation to detect the lesion. Aberrant proteins are therefore an obligate byproduct of mRNA surveillance and must be degraded to avoid disrupting protein homeostasis. These defective translation products are thought to be ubiquitinated at the ribosome, but the mechanism of ubiquitin ligase selectivity for these ribosomes is not clear. Here, we in vitro reconstitute ubiquitination of nascent proteins produced from aberrant mRNAs. Stalled 80S ribosome-nascent chain complexes are dissociated by the ribosome recycling factors Hbs1/Pelota/ABCE1 to a unique 60S-nascent chain-tRNA complex. The ubiquitin ligase Listerin preferentially recognizes 60S-nascent chains and triggers efficient nascent chain ubiquitination. Interfering with Hbs1 function stabilizes 80S complexes, precludes efficient Listerin recruitment, and reduces nascent chain ubiquitination. Thus, ribosome recycling factors control Listerin localization, explaining how translation products of mRNA surveillance are efficiently ubiquitinated while sparing translating ribosomes. Nascent protein ubiquitination on stalled ribosomes has been reconstituted in vitro Ubiquitination on separated 60S subunit is strongly preferred over intact 80S ribosome Nascent chain inside a 60S subunit cues stable binding of ubiquitin ligase Listerin Subunit dissociation by ribosome recycling factors enables ligase recruitment
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DOI：
10.1073/pnas.0812819106
发表时间：
2009-02-17
影响因子：
11.1
作者：
Chu, Jessie;Hong, Nancy A.;Kay, Steve A.
通讯作者：
Kay, Steve A.
影响因子：
4.8
作者：
Dimitrova, Lyudmila N.;Kuroha, Kazushige;Inada, Toshifumi
通讯作者：
Inada, Toshifumi
DOI：
10.1073/pnas.1113956108
发表时间：
2011-12-20
影响因子：
11.1
作者：
Shoemaker, Christopher J.;Green, Rachel
通讯作者：
Green, Rachel
影响因子：
64.8
作者：
Hessa, Tara;Sharma, Ajay;Mariappan, Malaiyalam;Eshleman, Heather D.;Gutierrez, Erik;Hegde, Ramanujan S.
通讯作者：
Hegde, Ramanujan S.
影响因子：
16.8
作者：
Shoemaker, Christopher J.;Green, Rachel
通讯作者：
Green, Rachel