Characterization of the guinea pig animal model and subsequent comparison of the behavioral effects of selective dopaminergic drugs and methamphetamine.
Characterization of the guinea pig animal model and subsequent comparison of the behavioral effects of selective dopaminergic drugs and methamphetamine.
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DOI：
10.1002/syn.21731
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发表时间：
2014-05
期刊：
影响因子：
2.3
通讯作者：
Chirwa, Sanika
中科院分区：
文献类型：
--
作者：
Lee, Kiera-Nicole;Pellom, Samuel T.;Oliver, Ericka;Chirwa, Sanika
虽然不常用于行为测试，但豚鼠可能提供更好地模仿人类活动的微妙行为，值得研究。为了验证这一点，使用视频跟踪EthoVision XT 7.0软件对PhenoTyper笼中的31只Hartley豚鼠（雄性，200-250 g）进行了评价。结果表明，豚鼠在隐蔽区（笼角的小盒子）的时间多于食物/水区或竞技场区。豚鼠表现出趋触性（墙以下的策略），并积极在整个光明和黑暗阶段。吃和喝发生在整个光明和黑暗阶段。注射0.25 mg/kg的多巴胺D1受体（D1 R）拮抗剂SCH 23390，在隐藏区花费的时间显着减少。接受7.5 mg SKF 38393（D1 R激动剂）、1.0 mg/kg舒必利（D2 R拮抗剂）和1.0或10.0 mg/kg甲基苯丙胺给药的豚鼠在隐藏区的时间变化不显著。注射生理盐水、SKF 38393、SCH 23390和舒必利后，自发活动曲线无变化。相比之下，单次注射或重复给药7天的低剂量甲基苯丙胺诱导短暂的多动症，但这下降到基线水平超过22小时的观察期。接受高剂量甲基苯丙胺治疗的豚鼠表现出持续的多动症，并在昼夜节律周期中行驶了更长的距离。随后7天的治疗与高剂量甲基苯丙胺诱导运动致敏和显着增加的总距离移动相对于单一药物注射或生理盐水对照。这些结果突出了豚鼠研究大脑行为相互作用的多功能性和独特性。
Though not commonly used in behavior tests guinea pigs may offer subtle behavior repertoires that better mimic human activity and warrant study. To test this, 31 Hartley guinea pigs (male, 200–250 g) were evaluated in PhenoTyper cages using the video-tracking EthoVision XT 7.0 software. Results showed that guinea pigs spent more time in the hidden zone (small box in corner of cage) than the food/water zone, or arena zone. Guinea pigs exhibited thigmotaxis (a wall following strategy) and were active throughout the light and dark phases. Eating and drinking occurred throughout the light and dark phases. An injection of 0.25 mg/kg SCH23390, the dopamine D1 receptors (D1R) antagonist, produced significant decreases in time spent in the hidden zone. There were insignificant changes in time spent in the hidden zone for guinea pigs treated with 7.5 mg SKF38393 (D1R agonist), 1.0 mg/kg sulpiride (D2R antagonist), and 1.0 or 10.0 mg/kg methamphetamine. Locomotor activity profiles were unchanged after injections of saline, SKF38393, SCH23390 and sulpiride. By contrast, a single injection or repeated administration for 7 days of low-dose methamphetamine induced transient hyperactivity but this declined to baseline levels over the 22-hour observation period. Guinea pigs treated with high-dose methamphetamine displayed sustained hyperactivity and travelled significantly greater distances over the circadian cycle. Subsequent 7-day treatment with high-dose methamphetamine induced motor sensitization and significant increases in total distances moved relative to single drug injections or saline controls. These results highlight the versatility and unique features of the guinea pig for studying brain-behavior interactions.
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影响因子：
2.7
作者：
ENNACEUR, A;DELACOUR, J
通讯作者：
DELACOUR, J
影响因子：
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作者：
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作者：
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作者：
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作者：
IBUKA, N
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IBUKA, N