High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia.
High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia.
复制标题
DOI：
10.1016/j.celrep.2018.03.114
复制
发表时间：
2018-04-24
期刊：
影响因子：
8.8
通讯作者：
Lorens JB
中科院分区：
文献类型：
--
作者：
Pelissier Vatter FA;Schapiro D;Chang H;Borowsky AD;Lee JK;Parvin B;Stampfer MR;LaBarge MA;Bodenmiller B;Lorens JB
Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. Aging human mammary tissue shows skewed progenitor cell potency, resulting in diminished tumor-suppressive cell types and the accumulation of defective epithelial progenitors. Quantitative characterization of these age-emergent human cell subpopulations is lacking, impeding our understanding of the relationship between age and cancer susceptibility. We conducted single-cell resolution proteomic phenotyping of healthy breast epithelia from 57 women, aged 16–91 years, using mass cytometry. Remarkable heterogeneity was quantified within the two mammary epithelial lineages. Population partitioning identified a subset of aberrant basal-like luminal cells that accumulate with age and originate from age-altered progenitors. Quantification of age-emergent phenotypes enabled robust classification of breast tissues by age in healthy women. This high-resolution mapping highlighted specific epithelial subpopulations that change with age in a manner consistent with increased susceptibility to breast cancer. CyTOF analysis reveals human mammary epithelial heterogeneity with age Age-emergent luminal cells share phenotypes with candidate breast cancer cells of origin Classification models correctly assign tissue samples to their age group Age-related changes are conserved between mammary epithelial tissue and primary cells Vatter et al. find that single-cell mass cytometry of human mammary epithelial cells from 57 women, from 16 to 91 years old, depicts an in-depth phenotyping of aging mammary epithelia. Subpopulations of altered luminal and progenitor cells that accumulate with age may be at increased risk for oncogenic transformation.
登录
查看更多内容
年龄和绕过停滞的手段会影响永生的人类乳腺上皮细胞的内在亚型。
影响因子：
5.5
作者：
Lee JK;Garbe JC;Vrba L;Miyano M;Futscher BW;Stampfer MR;LaBarge MA
通讯作者：
LaBarge MA
影响因子：
48
作者：
Giesen, Charlotte;Wang, Hao A. O.;Bodenmiller, Bernd
通讯作者：
Bodenmiller, Bernd
影响因子：
23.9
作者：
Choudhury, Sibgat;Almendro, Vanessa;Polyak, Kornelia
通讯作者：
Polyak, Kornelia
DOI：
10.1073/pnas.0909333107
发表时间：
2010-01-19
影响因子：
11.1
作者：
Gjerdrum, Christine;Tiron, Crina;Lorens, James B.
通讯作者：
Lorens, James B.
影响因子：
1.2
作者：
LaBarge, Mark A.;Garbe, James C.;Stampfer, Martha R.
通讯作者：
Stampfer, Martha R.