Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.
Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.
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DOI：
10.1186/1758-3284-2-5
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发表时间：
2010-02-22
期刊：
影响因子：
--
通讯作者：
Epstein AL
中科院分区：
文献类型：
--
作者：
Liebertz DJ;Lechner MG;Masood R;Sinha UK;Han J;Puri RK;Correa AJ;Epstein AL
头颈部鳞状细胞癌是一种侵袭性和致命性的恶性肿瘤。公开可用的细胞系大多是舌源性的，或者尚未仔细表征。新的HNSCC细胞系的详细表征是必要的，以便为研究人员提供一个具体的重点，建立他们的调查。USC-HN 1细胞系是从组织培养中的原发性上颌HNSCC活检外植体建立的。然后通过以下方法进一步表征永生化细胞：在裸鼠中进行异源移植;相关HNSCC生物标志物的免疫组织化学染色;表面标志物的流式细胞术;细胞遗传学核型分析;人乳头瘤病毒和EB病毒筛选;用于癌基因和细胞因子分析的qRT-PCR;活化、切割的Notch 1水平的研究;以及详细的35，000个基因微阵列分析。表征实验证实了USC-HN 1的人HNSCC起源，包括与原始肿瘤相似的表型。病毒筛查显示没有HPV或EBV感染，而蛋白质印迹显示活化的切割的Notch 1显著上调。USC-HN 1是一种新的永生化细胞系，来源于上颌骨HNSCC。表征研究表明，该细胞系是HNSCC来源的，并显示出许多与文献中先前报道的相同的标志物。USC-HN 1可用于公共研究，并将进一步研究HNSCC和开发新的治疗方式。
Head and neck squamous cell carcinoma (HNSCC) is an aggressive and lethal malignancy. Publically available cell lines are mostly of lingual origin, or have not been carefully characterized. Detailed characterization of novel HNSCC cell lines is needed in order to provide researchers a concrete keystone on which to build their investigations. The USC-HN1 cell line was established from a primary maxillary HNSCC biopsy explant in tissue culture. The immortalized cells were then further characterized by heterotransplantation in Nude mice; immunohistochemical staining for relevant HNSCC biomarkers; flow cytometry for surface markers; cytogenetic karyotypic analysis; human papillomavirus and Epstein-Barr virus screening; qRT-PCR for oncogene and cytokine analysis; investigation of activated, cleaved Notch1 levels; and detailed 35,000 gene microarray analysis. Characterization experiments confirmed the human HNSCC origin of USC-HN1, including a phenotype similar to the original tumor. Viral screening revealed no HPV or EBV infection, while western blotting displayed significant upregulation of activated, cleaved Notch1. USC-HN1, a novel immortalized cell line has been derived from a maxillary HNSCC. Characterization studies have shown that the cell line is of HNSCC origin and displays many of the same markers previously reported in the literature. USC-HN1 is available for public research and will further the investigation of HNSCC and the development of new therapeutic modalities.
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DOI：
10.1186/1758-3284-1-27
发表时间：
2009-07-14
期刊：
Head & neck oncology
影响因子：
--
作者：
Han J;Kioi M;Chu WS;Kasperbauer JL;Strome SE;Puri RK
通讯作者：
Puri RK
DOI：
10.1016/j.canrad.2004.06.003
发表时间：
2004-08-01
期刊：
Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique
影响因子：
--
作者：
Hermann, R M;Fuzesi, L;Schmidberger, H
通讯作者：
Schmidberger, H
影响因子：
4.8
作者：
Kaur, J;Ralhan, R
通讯作者：
Ralhan, R
影响因子：
11.5
作者：
Bergmann, Christoph;Strauss, Laura;Whiteside, Theresa L.
通讯作者：
Whiteside, Theresa L.
影响因子：
7.3
作者：
Schlecht, N. F.;Burk, R. D.;Belbin, T. J.
通讯作者：
Belbin, T. J.