Sporadic TP53 transition mutations in chronic cholecystitis are possibly linked to gallbladder carcinogenesis.
Sporadic TP53 transition mutations in chronic cholecystitis are possibly linked to gallbladder carcinogenesis.
复制标题
慢性胆囊炎中的散发性 TP53 转换突变可能与胆囊癌发生有关。
作者：
N. Yanagisawa;K. Yamashita;T. Kuba;I. Okayasu
背景/目的
众所周知，胆囊癌经常表现出TP 53以及K-ras基因突变。本研究采用PCR-SSCP和直接测序法对胆囊切除术后无胆囊癌的患者的胆汁上清液和组织标本进行TP 53基因的检测，以探讨胆囊癌的发生机制。
材料和方法
对294例胆囊炎患者中的18例进行了胆汁上清TP 53突变的SSCP筛查，并对其组织标本进行了SSCP和直接测序。
结果
11例（61%）非肿瘤性粘膜样本显示转移条带，7例（64%）证实突变。出乎意料的是，没有病例在胆汁和组织样本中显示相同的点突变。G：C到A：T的转变（被认为是零星突变）占主导地位（77%）。
结论
散在的TP 53转换突变在非肿瘤性病变，如严重的胆囊炎，表明慢性胆囊炎癌序列在胆囊癌发生的重要性。
BACKGROUND/AIM
Gallbladder cancers are well known to frequently exhibit TP53 as well as K-ras gene mutations. This study performed a TP53 gene investigation by PCR-SSCP and direct sequencing using both bile supernatants and tissue samples from cholecystectomy specimens lacking gallbladder cancer, in order to investigate gallbladder carcinogenesis.
MATERIALS AND METHODS
Eighteen out of 294 cases, mainly of cholecystitis, were extracted by screening SSCP of bile supernatants for TP53 mutations, and investigation of their tissue samples both SSCP and direct sequencing.
RESULTS
Non-neoplastic mucosal samples demonstrated shifted bands in 11 cases (61%), and mutations were confirmed in 7 cases (64%). Unexpectedly, no cases showed identical point mutations in both bile and tissue samples. G:C to A:T transitions, thought to be sporadic mutations, predominated (77%).
CONCLUSION
Sporadic TP53 transition mutations were demonstrated in non-neoplastic lesions such as severe cholecystitis, indicating an importance for a chronic cholecystitis-carcinoma sequence in gallbladder carcinogenesis.
影响因子：
11.2
作者：
T. Brentnall;David A. Crispin;M. Bronner;Sajeev P. Cherian;Molly Hueffed;P. Rabinovitch;C. Rubin;
通讯作者：
T. Brentnall;David A. Crispin;M. Bronner;Sajeev P. Cherian;Molly Hueffed;P. Rabinovitch;C. Rubin;
影响因子：
56.9
作者：
RIDEOUT, WM;COETZEE, GA;JONES, PA
通讯作者：
JONES, PA