TGF-β1 enhances contractility in engineered skeletal muscle.
TGF-β1 enhances contractility in engineered skeletal muscle.
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DOI：
10.1002/term.551
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发表时间：
2013-07
影响因子：
3.3
通讯作者：
Larkin, Lisa M.
中科院分区：
文献类型：
--
作者：
Weist, Michael R.;Wellington, Michael S.;Bermudez, Jacob E.;Kostrominova, Tatiana Y.;Mendias, Christopher L.;Arruda, Ellen M.;Larkin, Lisa M.
关键词：
由卫星细胞创建的无支架工程 3D 骨骼肌组织有可能替代因严重创伤或疾病而损失的肌肉组织。转化生长因子-β1 (TGF-β1) 在肌肉发育早期阶段介导卫星细胞的迁移和分化中发挥着至关重要的作用。此外，TGF-β1 促进骨骼肌细胞外基质 (ECM) 中 I 型胶原蛋白的合成，从而提供被动弹性基质来支撑肌纤维并促进力的传递。为了确定 TGF-β1 在体外骨骼肌构建体形成和收缩功能中的作用，我们创建了组织工程 3D 骨骼肌构建体，并在细胞培养基中添加了不同水平的重组 TGF-β1。在添加 TGF-β1 之前，原代细胞群由 75% Pax7 阳性细胞组成。与 0、0.5 和 1.0 ng/ml TGF-β1 相比，2.0 ng/ml TGF-β1 存在下，抽搐、破伤风和自发力的峰值力显着增加。使用 H&E 以及肌节肌球蛋白重链和 I 型胶原的免疫荧光染色对细胞结构进行可视化，结果显示，与 0、0.5 和 1.0 ng/ml 相比，2.0 ng/ml TGF-β1 存在下组织更好的肌纤维区域更密集。在工程化 3D 骨骼肌结构的培养基中添加 2.0 ng/ml TGF-β1 可增强收缩性和细胞外基质组织。
Scaffoldless engineered 3D skeletal muscle tissue created from satellite cells offers the potential to replace muscle tissue that is lost due to severe trauma or disease. Transforming growth factor-beta 1 (TGF-β1) plays a vital role in mediating migration and differentiation of satellite cells during the early stages of muscle development. Additionally, TGF-β1 promotes collagen type I synthesis in the extracellular matrix (ECM) of skeletal muscle, which provides a passive elastic substrate to support myofibres and facilitate the transmission of force. To determine the role of TGF-β1 in skeletal muscle construct formation and contractile function in vitro, we created tissue-engineered 3D skeletal muscle constructs with varying levels of recombinant TGF-β1 added to the cell culture medium. Prior to the addition of TGF-β1, the primary cell population was composed of 75% Pax7-positive cells. The peak force for twitch, tetanus and spontaneous force were significantly increased in the presence of 2.0 ng/ml TGF-β1 when compared to 0, 0.5 and 1.0 ng/ml TGF-β1. Visualization of the cellular structure with H&E and with immunofluorescence staining for sarcomeric myosin heavy chains and collagen type I showed denser regions of better organized myofibres in the presence of 2.0 ng/ml TGF-β1 versus 0, 0.5 and 1.0 ng/ml. The addition of 2.0 ng/ml TGF-β1 to the culture medium of engineered 3D skeletal muscle constructs enhanced contractility and extracellular matrix organization.
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影响因子：
33.6
作者：
Kontrogianni-Konstantopoulos A;Ackermann MA;Bowman AL;Yap SV;Bloch RJ
通讯作者：
Bloch RJ
影响因子：
2.4
作者：
Fauza, DO;Marler, JJ;Vacanti, JP
通讯作者：
Vacanti, JP
影响因子：
--
作者：
Larkin, Lisa M.;Calve, Sarah;Arruda, Ellen M.
通讯作者：
Arruda, Ellen M.
影响因子：
4.8
作者：
Duncan, MR;Frazier, KS;Grotendorst, GR
通讯作者：
Grotendorst, GR
影响因子：
4.6
作者：
Mathew, Sam J.;Hansen, Jody M.;Kardon, Gabrielle
通讯作者：
Kardon, Gabrielle