Vertical Transmission of the Zika Virus Causes Neurological Disorders in Mouse Offspring.
Vertical Transmission of the Zika Virus Causes Neurological Disorders in Mouse Offspring.
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寨卡病毒的垂直传播导致小鼠后代神经系统疾病
DOI：
10.1038/s41598-018-21894-w
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发表时间：
2018-02-23
影响因子：
4.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Shi Y;Li S;Wu Q;Sun L;Zhang J;Pan N;Wang Q;Bi Y;An J;Lu X;Gao GF;Wang X
寨卡病毒(ZIKV)感染与婴儿小头畸形等先天性畸形之间的关联是一种公共卫生紧急情况。虽然不同的活体模型被用于ZIKV的研究，但很少有动物模型可以用来研究母体ZIKV感染对新生儿发育的影响。在这里，我们通过宫内接种建立了一种具有免疫活性的小鼠模型。我们的结果证实，感染ZIKV而不是登革热病毒会导致小鼠后代自然流产、脑畸形、眼部畸形、脊髓缺陷和瘫痪。除了小头畸形和海马区发育不良外，感染ZIKV后还观察到眼睛异常，包括小眼球、视神经变细、视网膜受损和视觉投影不足。此外，感染寨卡病毒的后代表现出脊髓中阿尔法运动神经元的丢失和小脑畸形，这可能会导致瘫痪。ZIKV还损害了新生小鼠的成年神经发生。由于其完整的免疫力，我们的啮齿动物模型可以用来系统地评估ZIKV对胚胎和新生儿发育的影响，并探索潜在的治疗方法。
The association between Zika virus (ZIKV) infection and congenital malformations such as microcephaly in infants is a public health emergency. Although variousin vivoandin vitromodels are used for ZIKV research, few animal models are available for resolving the effects of maternal ZIKV infection on neonatal development. Here, we established an immunocompetent mouse model via intrauterine inoculation. Our results confirmed that ZIKV, but not dengue virus, infection caused spontaneous abortions, brain malformations, ocular abnormalities, spinal cord defects and paralysis in mouse offspring. Aside from microcephaly and hippocampal dysplasia, eye abnormalities, including microphthalmia, thinner optic nerves, damaged retinae, and deficient visual projection, were also observed following ZIKV infection. Moreover, ZIKV-infected offspring showed a loss of alpha motor neurons in the spinal cord and cerebellar malformation, which may cause paralysis. ZIKV also impaired adult neurogenesis in neonatal mice. Due to its intact immunity, our rodent model can be used to systematically evaluate the impact of ZIKV on embryonic and neonatal development and to explore potential therapies.
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影响因子：
30.3
作者：
Miner JJ;Diamond MS
通讯作者：
Diamond MS
影响因子：
8.8
作者：
Miner JJ;Sene A;Richner JM;Smith AM;Santeford A;Ban N;Weger-Lucarelli J;Manzella F;Rückert C;Govero J;Noguchi KK;Ebel GD;Diamond MS;Apte RS
通讯作者：
Apte RS
影响因子：
64.5
作者：
Bonaguidi MA;Wheeler MA;Shapiro JS;Stadel RP;Sun GJ;Ming GL;Song H
通讯作者：
Song H
DOI：
10.1056/nejmoa1602412
发表时间：
2016-12-15
期刊：
The New England journal of medicine
影响因子：
--
作者：
Brasil P;Pereira JP Jr;Moreira ME;Ribeiro Nogueira RM;Damasceno L;Wakimoto M;Rabello RS;Valderramos SG;Halai UA;Salles TS;Zin AA;Horovitz D;Daltro P;Boechat M;Raja Gabaglia C;Carvalho de Sequeira P;Pilotto JH;Medialdea-Carrera R;Cotrim da Cunha D;Abreu de Carvalho LM;Pone M;Machado Siqueira A;Calvet GA;Rodrigues Baião AE;Neves ES;Nassar de Carvalho PR;Hasue RH;Marschik PB;Einspieler C;Janzen C;Cherry JD;Bispo de Filippis AM;Nielsen-Saines K
通讯作者：
Nielsen-Saines K
影响因子：
168.9
作者：
Franca, Giovanny V. A.;Schuler-Faccini, Lavinia;Victora, Cesar G.
通讯作者：
Victora, Cesar G.