Terminal effects of optogenetic stimulation on dopamine dynamics in rat striatum.
Terminal effects of optogenetic stimulation on dopamine dynamics in rat striatum.
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DOI：
10.1016/j.jneumeth.2013.01.024
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发表时间：
2013-04-15
影响因子：
3
通讯作者：
Budygin, Evgeny A.
中科院分区：
文献类型：
--
作者：
Bass, Caroline E.;Grinevich, Valentina P.;Kulikova, Alexandra D.;Bonin, Keith D.;Budygin, Evgeny A.
在这项研究中，第一次深入分析的光学诱导多巴胺释放，使用快速扫描循环伏安法从大鼠大脑纹状体切片进行。将表达视紫红质-2的腺相关病毒注射到黑质中。4周后收集组织并切成400 μ m厚的冠状切片。蓝色激光（473 nm）通过插入切片组织的光纤传输。实验表明，从光学和电诱发多巴胺流出测量的最大幅度之间的一些差异。具体而言，有一个增加的幅度多巴胺释放诱导的电刺激相比，光刺激。然而，我们发现，多巴胺的释放是更敏感的光刺激的情况下的脉冲宽度的变化。随着刺激脉冲的加宽，光刺激多巴胺增加。刺激源之间以5分钟间隔重复刺激没有差异，并且多巴胺信号在记录会话期间稳定，而1分钟间隔导致两个源的振幅下降。光刺激也可以产生一种伪像，通过循环伏安图可以将其与多巴胺区分开来。这些结果证实，多巴胺的光学刺激是一个健全的方法，为未来的药理学研究切片。
In this study, the first in-depth analysis of optically induced dopamine release using fast-scan cyclic voltammetry on striatal slices from rat brain was performed. An adeno-associated virus that expresses Channelrhodopsin-2 was injected in the substantia nigra. Tissue was collected and sectioned into 400 μm-thick coronal slices four weeks later. Blue laser light (473 nm) was delivered through a fiber optic inserted into slice tissue. Experiments revealed some difference between maximal amplitudes measured from optically and electrically evoked dopamine effluxes. Specifically, there was an increase in the amplitude of dopamine release induced by electrical stimulation in comparison with light stimulations. However, we found that dopamine release is more sensitive to changes in the pulse width in the case of optical stimulation. Light-stimulated dopamine was increased as the stimulation pulse widened. There was no difference with repeated stimulations at five minute intervals between stimulation sources and dopamine signal was stable during recording sessions, while one minute intervals resulted in a decline in the amplitude from both sources. Optical stimulation can also produce an artifact that is distinguishable from dopamine by the cyclic voltammogram. These results confirm that optical stimulation of dopamine is a sound approach for future pharmacological studies in slices.
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影响因子：
7.7
作者：
LaLumiere, Ryan T.
通讯作者：
LaLumiere, Ryan T.
影响因子：
5
作者：
Budygin, EA;Mathews, TA;Jones, SR
通讯作者：
Jones, SR
影响因子：
3.3
作者：
通讯作者：
--
DOI：
10.1523/jneurosci.2246-11.2011
发表时间：
2011-07-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Adamantidis AR;Tsai HC;Boutrel B;Zhang F;Stuber GD;Budygin EA;Touriño C;Bonci A;Deisseroth K;de Lecea L
通讯作者：
de Lecea L
DOI：
10.1073/pnas.182412299
发表时间：
2002-09-03
影响因子：
11.1
作者：
Gao, GP;Alvira, MR;Wilson, JM
通讯作者：
Wilson, JM