Functional Dissection of Auxin Response Factors in Regulating Tomato Leaf Shape Development.
Functional Dissection of Auxin Response Factors in Regulating Tomato Leaf Shape Development.
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生长素响应因子在调节番茄叶形发育中的功能剖析。
DOI：
10.3389/fpls.2018.00957
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Wu L;Tian Z;Zhang J
植物激素生长素参与植物生长和发育过程的许多方面。番茄Aux/IAA转录因子SlIAA 9/ENTIRE/E在叶片形态发生和果实发育中起重要作用，并且E基因编码来自生长素反应阻遏物的Aux/IAA家族的蛋白。SlIAA 9-RNAi转基因和完整突变体植物都降低了番茄中的叶复杂性，但潜在的机制尚未完全解决。已知生长素信号通过Aux/IAA和ARF（auxin response factors）转录调节因子调节靶基因的表达。ARF介导广泛的发育过程。通过Y2 H（酵母双杂交）分析结合SlARF基因家族的表达谱，我们鉴定了一组ARF：SlARF 6A、SlARF 8A、SlARF 8B和SlARF 24。Pull-down和BiFC（双分子荧光互补）结果表明，这些SlARF在体外和体内与SlIAA 9相互作用，并且e突变改变了多种SlARF的表达模式。e突变体的单叶部分转化为野生型复叶的VIGS（病毒诱导的基因沉默），这四个SLARF。此外，IAA含量在这些样品中显着增加相比，e突变体。此外，SlARF 6A和SlARF 24与SlPIN 1启动子结合，并作为转录激活因子调节参与小叶起始的基因表达。这也可能表明，SlARF调节叶片形态，通过直接结合生长素响应基因的SlIAA 9的情况下，提供了一个洞察的作用，SlARF在叶片形状的发展。
The phytohormone auxin is involved in many aspects of plant growth and developmental processes. The tomato Aux/IAA transcription factor SlIAA9/ENTIRE/E plays an important role in leaf morphogenesis and fruit development, and the E gene encodes a protein from the Aux/IAA family of auxin response repressors. Both SlIAA9-RNAi transgenic and entire (e) mutant plants reduce the leaf complexity in tomato, but the underlying mechanism is not yet completely resolved. Auxin signaling is known to regulate target genes expression via Aux/IAA and ARFs (auxin response factors) transcriptional regulators. ARFs mediate a wide range of developmental processes. Through an Y2H (yeast two-hybrid) assay coupled with expression profiling of the SlARF genes family, we identified a group of ARFs: SlARF6A, SlARF8A, SlARF8B, and SlARF24. Pull-down and BiFC (Bimolecular Fluorescence Complementation) results demonstrated that these SlARFs interact with SlIAA9 in vitro and in vivo, and the e mutation altered the expression patterns of multiple SlARFs. The simple leaves of the e mutant were partially converted to wild-type compound leaves by VIGS (virus-induced gene silencing) of these four SlARFs. Furthermore, IAA content in these samples was significantly increased compared to the e mutant. In addition, SlARF6A and SlARF24 bound to the SlPIN1 promoter and act as transcriptional activators to regulate genes expression involved in leaflet initiation. It may also suggest that SlARFs regulate leaf morphology through direct binding to auxin-responsive genes in the absence of SlIAA9, providing an insight for the role of SlARFs in leaf shape development.
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