Structural studies on H-2Db and H-2Kd molecules indicate that murine major histocompatibility b and d haplotype alloantigens share structural features.-猫眼课题宝

H-2Db 和 H-2Kd 分子的结构研究表明，鼠主要组织相容性 b 和 d 单倍型同种抗原具有相同的结构特征。

DOI：

10.1016/0161-5890(80)90025-5

发表时间：

1980

期刊：

Molecular immunology

影响因子：

3.6

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

Kimball,ES;Maloy,WL;Martinko,JM;Nathenson,SG;Coligan,JE

通过放射化学方法检查了 H-2Dband H-2Kdmurine 主要组织相容性复合体 (MHC) 抗原的结构特性。通过分别对淋巴肿瘤细胞系 EL-4 (H-2b) 和 C14 (H-2d) 的 NP-40 裂解物进行间接免疫沉淀，获得 H-2D 带 H-2Kd 抗原的放射性标记制剂。制备好 37,000 分子量的木瓜蛋白酶片段后，用 CNBr 裂解抗原。 H-2Kd抗原产生四个主要CNBr片段，而H-2Db分子提供六个。对这些 CNBr 片段进行部分 NH2 末端氨基酸序列分析，并通过与 H-2Kb 糖蛋白的同源性进行比对。将 H-2Kd 和 H-2Db 分子的结构特性与先前公布的有关带状单倍型的其他已知主要移植抗原（H-2Kb、H-2Dd 和 H-2Ld）的数据进行比较，揭示了明显的结构相似性。首先，数据表明某些甲硫氨酸残基高度保守，这些位置上的 CNBr 裂解为研究这些分子提供了初步策略。其次，可以比对 CNBr 裂解后获得的二硫键连接的肽，并且数据表明在同源位置存在二硫键。第三，在CNBr裂解后，H-2Kd和H-2Db分子均产生与来自H-2Kb分子的糖肽同源的两种糖肽。第四，有限数量的可比位置的总体同源性在H-2Kband H-2Kdgene产品之间约为81％，在H-2Kband H-2Dbgene产品之间约为88％。

Structural properties of the H-2Dband H-2Kdmurine major histocompatibility complex (MHC) antigens were examined by radiochemical methods. Radiolabelled preparations of the H-2Dband H-2Kdantigens were obtained by indirect immune precipitation of NP-40 lysates of the lymphoid tumor cell lines EL-4 (H-2b) and C14 (H-2d), respectively. After preparation of the 37,000 molecular weight papain fragment the antigens were cleaved with CNBr. The H-2Kdantigen yielded four major CNBr fragments whereas the H-2Dbmolecule provided six. These CNBr fragments were subjected to partial NH2-terminal amino acid sequence analysis and aligned by homology to the H-2Kbglycoprotein. Comparison of the structural properties of the H-2Kdand H-2Dbmolecules with previously published data on the other known major transplantation antigens of thebanddhaplotypes (H-2Kb, H-2Ddand H-2Ld) reveal a marked structural similarity. First, the data show that certain methionine residues have been highly conserved and that cleavage by CNBr at these positions provides an initial strategy for the study of these molecules. Secondly, disulfide-linked peptides obtained after CNBr cleavage could be aligned and the data suggest the presence of disulfide bridges in homologous positions. Third, after CNBr cleavage both the H-2Kdand H-2Dbmolecules yielded two glycopeptides which were homologous to glycopeptides from the H-2Kbmolecule. Fourth, overall homology for a limited number of comparable positions is about 81% between the H-2Kband H-2Kdgene products and 88% between the H-2Kband H-2Dbgene products.