Nicotinic receptor Alpha7 expression during mouse adrenal gland development.
Nicotinic receptor Alpha7 expression during mouse adrenal gland development.
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DOI：
10.1371/journal.pone.0103861
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Rogers SW
中科院分区：
文献类型：
--
作者：
Gahring LC;Myers E;Palumbos S;Rogers SW
烟碱乙酰胆碱受体α 7（α7）是一种配体激活的离子通道，有助于参与发育、神经传递和炎症的多种细胞过程。在这份报告中，α7在小鼠发育和成年肾上腺中的表达进行了检查，该肾上腺表达绿色荧光蛋白（GFP）报告基因作为内源性α7转录本（α 7 G）的双顺反子延伸。在胚胎第12.5天（E12.5），α 7 G的表达与肾上腺的肾上腺神经节和前体细胞有关。α 7 G细胞是儿茶酚胺能嗜铬细胞，表现为酪氨酸羟化酶（TH）和多巴胺-β-羟化酶（DBH）的共表达逐渐增加，E18.5时完成。在成人中，α 7 G表达仅限于肾上腺髓质中聚集在肾上腺皮质边界附近的嗜铬细胞亚群。这些嗜铬细胞共表达α 7 G、TH和DBH，但它们缺乏苯乙醇胺N-甲基转移酶（PNMT），这与仅合成去甲肾上腺素（NE）一致。这些细胞群似乎优先受神经营养因子受体p75识别的节前传入神经支配。通过β-III微管蛋白、神经丝蛋白或p75共表达α 7 G，未鉴定出传入。在E14.5前胚胎中，偶尔有α 7 G细胞表达与内神经节细胞一致的神经元标记物，在成人中，一些α 7 G细胞共表达谷氨酸脱羧酶。肾上腺发育期间α7的瞬时表达及其在成人NE嗜铬细胞亚群中的显著共表达表明α7受体有助于肾上腺发育的多个方面和持续到成年的功能。
The nicotinic acetylcholine receptor alpha 7 (α7) is a ligand-activated ion channel that contributes to a diversity of cellular processes involved in development, neurotransmission and inflammation. In this report the expression of α7 was examined in the mouse developing and adult adrenal gland that expresses a green fluorescent protein (GFP) reporter as a bi-cistronic extension of the endogenous α7 transcript (α7G). At embryonic day 12.5 (E12.5) α7G expression was associated with the suprarenal ganglion and precursor cells of the adrenal gland. The α7G cells are catecholaminergic chromaffin cells as reflected by their progressive increase in the co-expression of tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) that is complete by E18.5. In the adult, α7G expression is limited to a subset of chromaffin cells in the adrenal medulla that cluster near the border with the adrenal cortex. These chromaffin cells co-express α7G, TH and DBH, but they lack phenylethanolamine N-methyltransferase (PNMT) consistent with only norepinephrine (NE) synthesis. These cell groups appear to be preferentially innervated by pre-ganglionic afferents identified by the neurotrophin receptor p75. No afferents identified by beta-III tubulin, neurofilament proteins or p75 co-expressed α7G. Occasional α7G cells in the pre-E14.5 embryos express neuronal markers consistent with intrinsic ganglion cells and in the adult some α7G cells co-express glutamic acid decarboxylase. The transient expression of α7 during adrenal gland development and its prominent co-expression by a subset of NE chromaffin cells in the adult suggests that the α7 receptor contributes to multiple aspects of adrenal gland development and function that persist into adulthood.
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