An improved surface passivation method for single-molecule studies.

An improved surface passivation method for single-molecule studies.
复制标题

DOI:
10.1038/nmeth.3143
复制
发表时间:
2014-12
期刊:
影响因子:
48
通讯作者:
Ha, Taekjip
Ha, Taekjip
中科院分区:
生物学1区
文献类型:
--
作者:
Hua, Boyang;Han, Kyu Young;Zhou, Ruobo;Kim, Hajin;Shi, Xinghua;Abeysirigunawardena, Sanjaya C.;Jain, Ankur;Singh, Digvijay;Aggarwal, Vasudha;Woodson, Sarah A.;Ha, Taekjip

文献摘要

参考文献

被引文献

相似文献

我们在此报告了一种用于体外单分子研究的表面钝化方法,与聚乙二醇表面相比,它更有效地防止生物分子的非特异性结合。这种新表面不会干扰系缚生物分子的行为和活动。它也可以用于在溶液中存在高浓度标记物种的单分子成像。减少准备时间和成本是另一个主要优势。
We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution. Reduction in preparation time and cost is another major advantage.
DOI: 10.1088/0034-4885/76/1/016601
发表时间: 2013-01
期刊: Reports on progress in physics. Physical Society (Great Britain)
影响因子: --
作者:
Kim H;Ha T
通讯作者: Ha T
DOI: 10.1074/jbc.m508684200
发表时间: 2005-12-23
影响因子: 4.8
作者:
Chen, YH;Kocherginskaya, SA;Cann, IKO
通讯作者: Cann, IKO
DOI: 10.1016/j.jcis.2009.09.007
发表时间: 2010-01-01
影响因子: 9.9
作者:
Jeyachandran, Y. L.;Mielczarski, J. A.;Rai, B.
通讯作者: Rai, B.
DOI: 10.1364/oe.21.001189
发表时间: 2013-01-14
期刊: OPTICS EXPRESS
影响因子: 3.8
作者:
Elting, Mary Williard;Leslie, Sabrina R.;Spudich, James A.
通讯作者: Spudich, James A.
DOI: 10.1101/gad.194936.112
发表时间: 2012-08-01
影响因子: 10.5
作者:
Revyakin, Andrey;Zhang, Zhengjian;Tjian, Robert
通讯作者: Tjian, Robert