An improved surface passivation method for single-molecule studies.
An improved surface passivation method for single-molecule studies.
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DOI:
10.1038/nmeth.3143
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发表时间:
2014-12
期刊:
影响因子:
48
通讯作者:
Ha, Taekjip
中科院分区:
文献类型:
--
作者:
Hua, Boyang;Han, Kyu Young;Zhou, Ruobo;Kim, Hajin;Shi, Xinghua;Abeysirigunawardena, Sanjaya C.;Jain, Ankur;Singh, Digvijay;Aggarwal, Vasudha;Woodson, Sarah A.;Ha, Taekjip
We herein report a surface passivation method for in vitro single-molecule studies, which more efficiently prevents non-specific binding of biomolecules as compared to the polyethylene glycol surface. The new surface does not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution. Reduction in preparation time and cost is another major advantage.
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DOI:
10.1088/0034-4885/76/1/016601
发表时间:
2013-01
期刊:
Reports on progress in physics. Physical Society (Great Britain)
影响因子:
--
作者:
Kim H;Ha T
通讯作者:
Ha T
影响因子:
4.8
作者:
Chen, YH;Kocherginskaya, SA;Cann, IKO
通讯作者:
Cann, IKO
影响因子:
9.9
作者:
Jeyachandran, Y. L.;Mielczarski, J. A.;Rai, B.
通讯作者:
Rai, B.
影响因子:
3.8
作者:
Elting, Mary Williard;Leslie, Sabrina R.;Spudich, James A.
通讯作者:
Spudich, James A.
影响因子:
10.5
作者:
Revyakin, Andrey;Zhang, Zhengjian;Tjian, Robert
通讯作者:
Tjian, Robert