Influence of Epidermal Thickness, Pigmentation and Redness on Skin Autofluorescence ¶
Influence of Epidermal Thickness, Pigmentation and Redness on Skin Autofluorescence ¶
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表皮厚度、色素沉着和发红对皮肤自发荧光的影响 ¶
DOI：
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发表时间：
2003
影响因子：
3.3
通讯作者：
H. Wulf
中科院分区：
文献类型：
--
作者：
Jane Sandby‐møller;T. Poulsen;H. Wulf
皮肤表面的自发荧光检测受黑色素和血红蛋白的影响很大。表皮的吸收和散射也可能是一个影响因素，在本文中被表示为一个定量参数，即表皮厚度。为了检验这一参数，我们测量了330 nm激发后的体内370 nm荧光和330和370 nm激发后的455 nm荧光。测量对象为前臂和肩部背侧暴露于阳光下的皮肤，以及未暴露于臀部的皮肤。用反射光谱仪测量同一部位皮肤的色素沉着和发红程度。皮肤活检光镜观察角质层和表皮细胞部的厚度。用多元回归分析寻找自体荧光与潜在影响因素之间的相关性。我们发现皮肤的自发荧光与两个发射波长(Em)的色素沉着和发红有非常显著的相关性。只有激发波长(Ex)370 nm和Em455 nm与表皮厚度有很小但显著的相关性，如果分析中包括身体部位的话。Ex330：Em370和Ex330：Em455与表皮厚度无相关性。在实际应用中，对皮肤色素沉着的自发荧光校正是必要的，对红色的校正是次要的，对表皮厚度的校正是不必要的。
Detection of autofluorescence at the skin surface is highly influenced by melanin and hemoglobin. Epidermal absorption and scattering may also be an influencing factor and is represented in this article as a quantitative parameter, epidermal thickness. To examine this parameter we measured the 370 nm fluorescence in vivo after excitation with 330 nm and the 455 nm fluorescence after excitation with 330 and 370 nm. Measurements were performed on sun‐exposed skin at the dorsal aspect of the forearm and shoulder and on nonexposed buttock skin. Skin pigmentation and redness of the same body sites were measured by reflectance spectroscopy. The thickness of the stratum corneum and the cellular part of epidermis was quantified by light microscopy of skin biopsies. Multiple regression analysis was used to find correlations between autofluorescence and the potential influencing factors. We found a highly significant correlation of skin autofluorescence with pigmentation and redness for both emission wavelengths (Em). A small but significant correlation to epidermal thickness was found only for excitation wavelength (Ex) 370 nm and Em455 nm if body site was included in the analysis. No correlation between Ex330:Em370 and Ex330:Em455 and thickness of epidermis was found. For practical use, correction of skin autofluorescence for pigmentation is essential, correction for redness is of less importance and correction for epidermal thickness is unnecessary.
影响因子：
2.9
作者：
D. Sell;V. Monnier
通讯作者：
D. Sell;V. Monnier
DOI：
10.1073/pnas.81.2.583
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MONNIER, VM;KOHN, RR;CERAMI, A
通讯作者：
CERAMI, A
影响因子：
6.5
作者：
ANDERSON, RR;PARRISH, JA
通讯作者：
PARRISH, JA