Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams.

Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams.
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DOI:
10.1364/oe.16.020734
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发表时间:
2008-12-08
期刊:
影响因子:
3.8
通讯作者:
Gao BZ
Gao BZ
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Moh KJ;Yuan XC;Bu J;Zhu SW;Gao BZ

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我们展示了表面等离子体共振传感和成像的概念验证,通过一个虚拟的探针在水介质中的生物细胞的细胞-基底界面。该技术是基于光学激发聚焦径向偏振光束的局部表面等离子体,形成一个虚拟的金属基板上的探针。在物镜透镜的后焦平面处的强度分布使得能够对细胞-基底接触进行定量测量。然后以局部折射率图的形式可视化所采集的数据。
We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which forms a virtual probe on the metal substrate. The intensity distribution at the back focal plane of the objective lens enables quantitative measurements to be made of the cell-substrate contact. The acquired data is then visualized in the form of a local refractive index map.
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