Herbal Compound "Songyou Yin" Renders Hepatocellular Carcinoma Sensitive to Oxaliplatin through Inhibition of Stemness.
Herbal Compound "Songyou Yin" Renders Hepatocellular Carcinoma Sensitive to Oxaliplatin through Inhibition of Stemness.
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DOI：
10.1155/2012/908601
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Tang ZY
中科院分区：
文献类型：
--
作者：
Jia QA;Ren ZG;Bu Y;Wang ZM;Zhang QB;Liang L;Jiang XM;Zhang QB;Tang ZY
研究复方松油饮对肝癌干细胞的影响。MHCC97H和Hep3B细胞株经SYY预处理4周后，评估其对奥沙利铂的化学敏感性。同时检测了csc相关标志物的表达、细胞的侵袭和迁移以及集落的形成。建立syy治疗的人肝癌原位裸鼠模型，探讨奥沙利铂对肿瘤生长、转移和生存的影响。我们还评估了csc在体内的相关分子变化。结果显示，SYY预处理的MHCC97H和Hep3B细胞对奥沙利铂的化学敏感性显著增加，scs相关标志物CD90、CD24和EPCAM下调。SYY还能减弱MHCC97H和Hep3B细胞系的细胞活力、侵袭和集落形成。syy预处理的细胞株的致瘤性和肺转移均降低。与单用奥沙利铂相比，奥沙利铂与SYY联合治疗可显著减少肿瘤体积和肺转移，延长生存期。免疫组化分析显示，联合治疗后，HCC细胞中CD90、ABCG2、ALDH、CD44、EPCAM、vimentin和MMP-9的表达降低，E-cadherin的表达升高。这些数据清楚地表明，SYY通过抑制干细胞使肝细胞癌对奥沙利铂敏感。
We investigated the effect of Chinese herbal compound Song-you Yin on HCC stemness. MHCC97H and Hep3B cell lines were pretreated with SYY for 4 weeks, and their chemosensitivity to oxaliplatin was evaluated. The expression of CSC-related markers, cell invasion and migration, and colony formation were also examined. SYY-treated orthotopic nude mouse models of human HCC were developed to explore the effect of oxaliplatin on tumor growth, metastasis, and survival. The CSC-related molecular changes in vivo were also evaluated. The result showed that MHCC97H and Hep3B cells pretreated with SYY showed significantly increased chemosensitivity to oxaliplatin and the downregulation of CSC-related markers CD90, CD24, and EPCAM. SYY also attenuated cell motility, invasion, and colony formation in MHCC97H and Hep3B cell lines. The reduced tumorigenicity and pulmonary metastasis were observed in SYY-pretreated cell lines. Combination treatment with oxaliplatin and SYY significantly reduced tumor volume and pulmonary metastasis and prolonged survival compared with oxaliplatin treatment alone. Immunohistochemical analysis showed reduced expression of CD90, ABCG2, ALDH, CD44, EPCAM, vimentin, and MMP-9 and increased the expression of E-cadherin, in HCC cells following combination treatment. These data clearly demonstrate that SYY renders hepatocellular carcinoma sensitive to oxaliplatin through the inhibition of stemness.
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影响因子：
23.9
作者：
Lee, Terence Kin Wah;Castilho, Antonia;Irene Oi Lin Ng
通讯作者：
Irene Oi Lin Ng
DOI：
10.1007/s12015-008-9037-x
发表时间：
2008-09-01
期刊：
STEM CELL REVIEWS
影响因子：
--
作者：
Rajan, Prithi;Srinivasan, Roopa
通讯作者：
Srinivasan, Roopa
影响因子：
11.2
作者：
Santisteban M;Reiman JM;Asiedu MK;Behrens MD;Nassar A;Kalli KR;Haluska P;Ingle JN;Hartmann LC;Manjili MH;Radisky DC;Ferrone S;Knutson KL
通讯作者：
Knutson KL
影响因子：
29.4
作者：
Carr, BI
通讯作者：
Carr, BI
DOI：
10.4161/cc.8.6.7907
发表时间：
2009-03-15
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Peter ME
通讯作者：
Peter ME