Inner nuclear membrane protein LEM-2 is required for correct nuclear separation and morphology in C. elegans
Inner nuclear membrane protein LEM-2 is required for correct nuclear separation and morphology in C. elegans
复制标题
线虫中正确的核分离和形态需要内核膜蛋白 LEM-2
作者：
A. Morales;A. Dobrzynska;P. Askjaer
摘要内核膜蛋白Emerin和LEMD2在调节核组织、基因表达和细胞分化方面既有重叠又有分离的功能。在此，我们报道了在秀丽线虫发育过程中的所有组织中都有Emerin(EMR-1)和LEM结构域蛋白2(LEM-2)的表达，但它们的相对分布在不同的细胞类型中是不同的。EMR-1/LEM-2的比值在收缩组织中最高，在神经元和皮下组织中居中，在肠道和胚系中最低。我们发现LEM-2比EMR-1更早被招募来重塑核膜，这表明存在单独的有丝分裂膜室和每个蛋白质的特定功能。一致地，我们观察到lem-2突变胚胎的核，而不是emr-1突变胚胎的核，减少了核的环形性。最后，我们揭示了LEM-2在微管组织中心的核分离和锚定中的一个迄今未知的作用。
ABSTRACT The inner nuclear membrane proteins emerin and LEMD2 have both overlapping and separate functions in regulation of nuclear organization, gene expression and cell differentiation. We report here that emerin (EMR-1) and LEM domain protein 2 (LEM-2) are expressed in all tissues throughout Caenorhaditis elegans development but their relative distribution differs between cell types. The ratio of EMR-1 to LEM-2 is particularly high in contractile tissues, intermediate in neurons and hypodermis and lowest in intestine and germ line. We find that LEM-2 is recruited earlier than EMR-1 to reforming nuclear envelopes, suggesting the presence of separate mitotic membrane compartments and specific functions of each protein. Concordantly, we observe that nuclei of lem-2 mutant embryos, but not of emr-1 mutants, have reduced nuclear circularity. Finally, we uncover a so-far-unknown role of LEM-2 in nuclear separation and anchoring of microtubule organizing centers.
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影响因子：
4
作者：
T. Haraguchi;T. Koujin;M. Segura-Totten;Kenneth K. Lee;Y. Matsuoka;Y. Yoneda;K. Wilson;Y. Hiraoka
通讯作者：
T. Haraguchi;T. Koujin;M. Segura-Totten;Kenneth K. Lee;Y. Matsuoka;Y. Yoneda;K. Wilson;Y. Hiraoka
影响因子：
3.3
作者：
Lee, KK;Gruenbaum, Y;Wilson, KL
通讯作者：
Wilson, KL
影响因子：
7.5
作者：
Gomez-Cavazos, J. Sebastian;Hetzer, Martin W.
通讯作者：
Hetzer, Martin W.
DOI：
10.1146/annurev-cellbio-100109-104037
发表时间：
2010
影响因子：
11.3
作者：
Starr DA;Fridolfsson HN
通讯作者：
Fridolfsson HN
线虫中 emerin 的表达、核纤层蛋白依赖性定位和 RNAi 耗竭表型。
影响因子：
4
作者：
Gruenbaum,Yosef;Lee,KennethK;Liu,Jun;Cohen,Merav;Wilson,KatherineL
通讯作者：
Wilson,KatherineL