The crystal structure of Pyrococcus furiosus RecJ implicates it as an ancestor of eukaryotic Cdc45.
The crystal structure of Pyrococcus furiosus RecJ implicates it as an ancestor of eukaryotic Cdc45.
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强烈火球菌 RecJ 的晶体结构表明它是真核生物 Cdc45 的祖先
DOI：
10.1093/nar/gkx887
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发表时间：
2017-12-01
影响因子：
14.9
通讯作者：
Liu XP
中科院分区：
文献类型：
--
作者：
Li MJ;Yi GS;Yu F;Zhou H;Chen JN;Xu CY;Wang FP;Xiao X;He JH;Liu XP
RecJ nucleases specifically degrade single-stranded (ss) DNA in the 5′ to 3′ direction. Archaeal RecJ is different from bacterial RecJ in sequence, domain organization, and substrate specificity. The RecJ from archaea Pyrococcus furiosus (PfuRecJ) also hydrolyzes RNA strands in the 3′ to 5′ direction. Like eukaryotic Cdc45 protein, archaeal RecJ forms a complex with MCM helicase and GINS. Here, we report the crystal structures of PfuRecJ and the complex of PfuRecJ and two CMPs. PfuRecJ bind one or two divalent metal ions in its crystal structure. A channel consisting of several positively charged residues is identified in the complex structure, and might be responsible for binding substrate ssDNA and/or releasing single nucleotide products. The deletion of the complex interaction domain (CID) increases the values of kcat/Km of 5′ exonuclease activity on ssDNA and 3′ exonuclease activity on ssRNA by 5- and 4-fold, respectively, indicating that the CID functions as a regulator of enzymatic activity. The DHH domain of PfuRecJ interacts with the C-terminal beta-sheet domain of the GINS51 subunit in the tetrameric GINS complex. The relationship of archaeal and bacterial RecJs, as well as eukaryotic Cdc45, is discussed based on biochemical and structural results.
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影响因子：
7.7
作者：
Cheng K;Xu H;Chen X;Wang L;Tian B;Zhao Y;Hua Y
通讯作者：
Hua Y
影响因子：
64.5
作者：
Hopkins BB;Paull TT
通讯作者：
Paull TT
影响因子：
14.9
作者：
Oyama T;Ishino S;Shirai T;Yamagami T;Nagata M;Ogino H;Kusunoki M;Ishino Y
通讯作者：
Ishino Y
影响因子：
21.3
作者：
Gambus, A;Jones, RC;Labib, K
通讯作者：
Labib, K
DOI：
10.1007/978-1-4939-2596-4_13
发表时间：
2015-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Giroux, Xavier;MacNeill, Stuart A
通讯作者：
MacNeill, Stuart A