Characterizing the detection threshold for optical imaging in surgical oncology.
Characterizing the detection threshold for optical imaging in surgical oncology.
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DOI：
10.1002/jso.24733
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发表时间：
2017-12
影响因子：
2.5
通讯作者：
Warram JM
中科院分区：
文献类型：
--
作者：
Prince AC;Jani A;Korb M;Tipirneni KE;Kasten BB;Rosenthal EL;Warram JM
引导癌症切除的光学成像正在迅速过渡到手术室。然而，该技术检测亚临床疾病的灵敏度尚未确定。本研究的目的是确定可以通过基于抗体的荧光成像检测到的癌细胞的最小范围。将与西妥昔单抗-IRDye 800 CW（剂量范围8.6μM至86 nM）体外孵育的2LMP（乳腺）、科洛-205（结肠）、MiaPaca-2（胰腺）和SCC-1（头颈部）细胞皮下植入小鼠（n=3只小鼠，5个肿瘤/小鼠）。在体外与8.6×10−2μM西妥昔单抗-IRDye 800 CW孵育后，将每种细胞类型的系列稀释液（1×103-1×106）皮下植入（n=3，5个肿瘤/小鼠）。用Pearl Impulse和Xenogen IVIS 100成像系统对肿瘤进行成像。进行Scatchard分析以确定每个细胞系的受体密度和动力学。在最低限度的西妥昔单抗-IRDye 800 CW暴露于低细胞量的条件下，闭场荧光成像理论上可检测到至少4.2×104-9.5×104个2LMP细胞、1.9×105-4.5×105个MiaPaca-2细胞和2.4×104-6.7×104个SCC-1细胞;无法识别科洛-205细胞。EGFR介导的西妥昔单抗高摄取与检测灵敏度相关。本研究支持西妥昔单抗-IRDye 800 CW在手术环境中敏感定位亚临床疾病的临床效用。
Optical imaging to guide cancer resections is rapidly transitioning into the operating room. However, the sensitivity of this technique to detect subclinical disease is yet characterized. The purpose of this study was to determine the minimum range of cancer cells that can be detected by antibody-based fluorescence imaging. 2LMP (breast), COLO-205 (colon), MiaPaca-2 (pancreas), and SCC-1 (head and neck) cells incubated in vitro with cetuximab-IRDye800CW (dose range 8.6μM to 86nM) were implanted subcutaneously in mice (n=3 mice, 5 tumors/mouse). Following incubation with 8.6×10−2μM of cetuximab-IRDye800CW in vitro, serial dilutions of each cell type (1×103–1×106) were implanted subcutaneously (n=3, 5 tumors/mouse). Tumors were imaged with Pearl Impulse and Xenogen IVIS 100 imaging systems. Scatchard analysis was performed to determine receptor density and kinetics for each cell line. Under conditions of minimal cetuximab-IRDye800CW exposure to low cellular quantity, closed-field fluorescence imaging theoretically detected a minimum of 4.2×104–9.5×104 2LMP cells, 1.9×105–4.5×105 MiaPaca-2 cells, and 2.4×104–6.7×104 SCC-1 cells; COLO-205 cells could not be identified. Higher EGFR-mediated uptake of cetuximab correlated with sensitivity of detection. This study supports the clinical utility of cetuximab-IRDye800CW to sensitively localize subclinical disease in the surgical setting.
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影响因子：
3.1
作者：
Marshall, Milton V.;Draney, Daniel;Sevick-Muraca, Eva M.;Olive, D. Michael
通讯作者：
Olive, D. Michael
影响因子：
4.7
作者：
TWAALFHOVEN, FCM;PETERS, AAW;FLEUREN, GJ
通讯作者：
FLEUREN, GJ
DOI：
10.1016/0360-3016(95)02065-9
发表时间：
1996-01-15
影响因子：
7
作者：
Groebe, K;MuellerKlieser, W
通讯作者：
MuellerKlieser, W
影响因子：
4.3
作者：
Naumov, George N.;Akslen, Lars A.;Folkman, Judah
通讯作者：
Folkman, Judah
影响因子：
2.9
作者：
Sorace, Anna G.;Korb, Melissa;Warram, Jason M.;Umphrey, Heidi;Zinn, Kurt R.;Rosenthal, Eben;Hoyt, Kenneth
通讯作者：
Hoyt, Kenneth