Over-expression of chrysanthemum CmDREB6 enhanced tolerance of chrysanthemum to heat stress.
Over-expression of chrysanthemum CmDREB6 enhanced tolerance of chrysanthemum to heat stress.
复制标题
菊花CmDREB6过表达增强菊花对热应激的耐受性
DOI：
10.1186/s12870-018-1400-8
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发表时间：
2018-09-04
影响因子：
5.3
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Du X;Li W;Sheng L;Deng Y;Wang Y;Zhang W;Yu K;Jiang J;Fang W;Guan Z;Chen F;Chen S
菊花是中国十大传统花卉之一，也是世界四大切花之一，但高温严重限制了菊花的生长，阻碍了菊花的生长，造成花的缺陷。DREB(脱水反应元件结合)转录因子在对非生物和生物胁迫的响应中发挥着重要作用。然而，DREB A-6亚组是否与耐热性有关尚未有定论。本研究从菊花‘金巴’中克隆了CmDREB6基因。CmDREB6含有一个典型的AP2/ERF结构域，属于DREB A-6亚组，与菊苣的同源性最高(73%)。CmDREB6在叶片中的表达水平最高。CmDREB6蛋白定位于细胞核。根据酵母单杂交实验，CmDREB6在酵母中具有转录激活活性，转录激活区域位于3‘端，范围为230-289个氨基酸残基。CmDREB6过表达提高了菊花的耐热性。两个转基因株系的存活率分别高达85%和50%，而野生型(WT)的存活率为3.8%。CmDREB6的过表达促进了CmHsfA4、CmHSP90以及活性氧清除基因CmSOD和CmCAT的表达。本研究从菊花‘金巴’中克隆了DREB A-6亚群基因CmDREB6。过表达CmDREB6通过调节参与热休克反应和ROS同源发生的基因来提高菊花的耐热性。
Chrysanthemum is among the top ten traditional flowers in China, and one of the four major cut flowers in the world, but the growth of chrysanthemum is severely restricted by high temperatures which retard growth and cause defects in flowers. DREB (dehydration-responsive element-binding) transcription factors play important roles in the response to abiotic and biotic stresses. However, whether the DREB A-6 subgroup is involved in heat tolerance has not been reported conclusively. In the present study, CmDREB6 was cloned from chrysanthemum (Chrysanthemum morifolium) ‘Jinba’. CmDREB6, containing a typical AP2/ERF domain, was classed into the DREB A-6 subgroup and shared highest homology with Cichorium intybus L. CiDREB6 (73%). CmDREB6 was expressed at its highest levels in the leaf. The CmDREB6 protein localized to the nucleus. Based on the yeast one hybrid assay, CmDREB6 showed transcription activation activity in yeast, and the transcriptional activation domain was located in the 3 ‘end ranging from 230 to 289 amino acids residues. CmDREB6 overexpression enhanced the tolerance of chrysanthemum to heat. The survival rate of two transgenic lines was as high as 85%, 50%, respectively, in contrast to 3.8% of wild-type (WT). Over-expression of CmDREB6 promoted the expression of CmHsfA4, CmHSP90, and the active oxygen scavenging genes CmSOD and CmCAT. In this study, DREB A-6 subgroup gene CmDREB6 was cloned from chrysanthemum ‘Jinba’. Overexpression of CmDREB6 enhanced heat tolerance of chrysanthemum by regulating genes involved in the heat shock response and ROS homeogenesis.
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影响因子：
5.1
作者：
Schramm, F;Ganguli, A;von Koskull-Döring, P
通讯作者：
von Koskull-Döring, P
影响因子：
4.8
作者：
Basha, Eman;Jones, Christopher;Vierling, Elizabeth
通讯作者：
Vierling, Elizabeth
DOI：
10.1016/j.bbrc.2010.09.038
发表时间：
2010-10-15
影响因子：
3.1
作者：
Chen, Huan;Hwang, Jung Eun;Lim, Chae Oh
通讯作者：
Lim, Chae Oh
CgDREBa 转基因菊花赋予干旱和盐度耐受性
影响因子：
5.7
作者：
Chen, Sumei;Cui, Xinli;Fang, Weimin
通讯作者：
Fang, Weimin
影响因子：
7.4
作者：
Perez-Salamo, Imma;Papdi, Csaba;Szabados, Laszlo
通讯作者：
Szabados, Laszlo