Microarray-based transcriptomic analysis of differences between long-term gregarious and solitarious desert locusts.
Microarray-based transcriptomic analysis of differences between long-term gregarious and solitarious desert locusts.
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基于微阵列的转录组分析长期群居和独居沙漠蝗虫之间的差异。
DOI：
10.1371/journal.pone.0028110
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Vanden Broeck J
中科院分区：
文献类型：
--
作者：
Badisco L;Ott SR;Rogers SM;Matheson T;Knapen D;Vergauwen L;Verlinden H;Marchal E;Sheehy MR;Burrows M;Vanden Broeck J
沙漠蝗（Schistocerca gregaria）表现出一种极端的表型可塑性，可以在隐蔽的独居阶段和群居阶段之间转换。这两个阶段在行为、形态和生理上有很大的不同，但对这些差异的分子基础知之甚少。本研究利用最近建立的表达序列标签（EST）数据库，设计了寡核苷酸微阵列，并比较了长期群居和独居的成年沙漠蝗虫中枢神经系统中数千个基因的表达。鉴定出214个差异表达基因，其中40%已被标注。这些基因包括编码与中枢神经系统发育和建模、感觉知觉、应激反应和抵抗以及基本细胞过程相关的蛋白质的基因。我们的微阵列分析已经确定了表达改变的基因，这些基因可能使蝗虫能够应对它们所面临的不同挑战。在群居蝗虫中，热休克蛋白和保护免受感染的蛋白的基因被上调，这可能使它们能够对急性生理挑战做出反应。相比之下，寿命较长的独居蝗虫似乎受到了更强的保护，免受与抗氧化系统、解毒和合成代谢更新有关的基因的上调，从而免受衰老缓慢累积的影响。群居蝗虫也有更丰富的参与感觉加工和神经系统发育和可塑性的蛋白质转录本。群居的蝗虫比独居的蝗虫生活在一个更复杂的感官环境中，可能需要更多的涉及感觉转导的蛋白质周转，并且可能需要更大的神经元可塑性。
Desert locusts (Schistocerca gregaria) show an extreme form of phenotypic plasticity and can transform between a cryptic solitarious phase and a swarming gregarious phase. The two phases differ extensively in behavior, morphology and physiology but very little is known about the molecular basis of these differences. We used our recently generated Expressed Sequence Tag (EST) database derived from S. gregaria central nervous system (CNS) to design oligonucleotide microarrays and compare the expression of thousands of genes in the CNS of long-term gregarious and solitarious adult desert locusts. This identified 214 differentially expressed genes, of which 40% have been annotated to date. These include genes encoding proteins that are associated with CNS development and modeling, sensory perception, stress response and resistance, and fundamental cellular processes. Our microarray analysis has identified genes whose altered expression may enable locusts of either phase to deal with the different challenges they face. Genes for heat shock proteins and proteins which confer protection from infection were upregulated in gregarious locusts, which may allow them to respond to acute physiological challenges. By contrast the longer-lived solitarious locusts appear to be more strongly protected from the slowly accumulating effects of ageing by an upregulation of genes related to anti-oxidant systems, detoxification and anabolic renewal. Gregarious locusts also had a greater abundance of transcripts for proteins involved in sensory processing and in nervous system development and plasticity. Gregarious locusts live in a more complex sensory environment than solitarious locusts and may require a greater turnover of proteins involved in sensory transduction, and possibly greater neuronal plasticity.
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DOI：
10.1046/j.1432-1327.1999.00438.x
发表时间：
1999-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Angeli, S;Ceron, F;Pelosi, P
通讯作者：
Pelosi, P
影响因子：
56.9
作者：
Goll, MG;Kirpekar, F;Bestor, TH
通讯作者：
Bestor, TH
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.7
作者：
Djagaeva I;Doronkin S
通讯作者：
Doronkin S