Original Article Enumeration of monocytes subsets using different gating methods by flow cytometry
Original Article Enumeration of monocytes subsets using different gating methods by flow cytometry
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原创文章通过流式细胞术使用不同门控方法对单核细胞亚群进行计数
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Published February
中科院分区：
文献类型：
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作者：
Hua Wang;Saili Wang;Jun Ye;Yong Yin;Yupei Zhou;Xiao;Yan Wang;R. November;Accepted January;Epub February;Published February
目的：比较流式细胞仪计数单核细胞亚群时不同设门方法的效率。方法：随机选择在我院体检的健康体检者48例。采用流式细胞术检测外周血单核细胞亚群，采用FSC-SSC、CD 45和CD 14设门方法。此外，还分析了不同贮存时间对检测结果的影响。结果：3种不同设门方法获得的Mon 1和Mon 3计数值差异有统计学意义（P0.05）。CD 45设门法测定的Mon 2细胞百分率与CD 14设门法比较有显著性差异。相关性检验显示，三种设门方法对Mon 1、Mon 2、Mon 3的检测呈显著正相关（P<0. 05）。对于储存时间对检测结果的影响，与CD 14设门在1 h时相比，FSC-SSC设门在8 h时测定的Mon 2百分比显著增加。FSC-SSC设门后8h Mon 3表达较CD 14设门后1h明显降低（P<0.05）。与4 h相比，8h时SSC低细胞群中Mon 1、Mon 2和Mon 3的比例差异有统计学意义（P<0.05）。对于在室温下储存4 h的样品，与SSC高相比，SSC低中的Mon 1和Mon 3比例存在显著差异。对于室温下储存8 h的样品，与SSC高相比，SSC低中的Mon 1、Mon 2和Mon 3比例观察到显著差异。结论：FSC-SSC设门或CD 45设门是Mon 2的有效计数方法。CD 14门控更适合于Mon 1的计数。由于储存时间的延长可能会影响单核细胞亚群的分析，临床分析样本应尽可能及时检测。
Objective: To compare the efficiency of different gating methods for the enumeration of monocytes sub - sets using flow cytometry. Methods: Forty-eight healthy individuals received physical examination in our hospital was randomly included in this study. Peripheral blood monocytes subsets were analyzed by flow cytometry with FSC-SSC, CD45 and CD14 gating methods. Besides, the effects of different storage times on the test results were analyzed. Results: Statistical difference was noticed in the enumeration of Mon1 and Mon3 obtained using three different gating methods (P 0.05). The percentage of Mon2 determined using CD45 gating showed remarkable difference compared with that of CD14 gating. Correlation test showed that the three gating methods for Mon1, Mon2, Mon3 detection showed a significant positive correlation ( P<0.05). For the effects of storage duration on the test results, significant increase was noticed in the percentage of Mon2 determined by FSC-SSC gating at 8 h compared with that of CD14 gating at 1 h. Nevertheless, remarkable decrease was identified in the Mon3 by FSC-SSC gating at 8 h compared with that of CD14 gating at 1 h (P<0.05). Significant difference was observed in the Mon1, Mon2 and Mon3 proportion in SSC low cell populations at 8 h compared with those obtained at 4 h (P<0.05). For the samples stored at room temperature for 4 h, significant differences were identified in the Mon1 and Mon3 proportion in SSC low compared with those of the SSC hi . For the samples stored at room temperature for 8 h, significant differences were observed in the Mon1, Mon2 and Mon3 proportion in SSC low compared with those of SSC hi . Conclusion: FSC-SSC gating or CD45 gating was effective for the enumeration of Mon2. CD14 gat- ing is more suitable for the enumeration of Mon1. As the extension of storage duration may affect the analysis of monocytes subsets, clinical samples for analysis should be tested as timely as possible.
影响因子：
37.8
作者：
Nahrendorf M;Pittet MJ;Swirski FK
通讯作者：
Swirski FK
影响因子：
6.7
作者：
Hristov, Mihail;Weber, Christian
通讯作者：
Weber, Christian