Rapid Macrosatellite Evolution Promotes X-Linked Hybrid Male Sterility in a Feline Interspecies Cross.
Rapid Macrosatellite Evolution Promotes X-Linked Hybrid Male Sterility in a Feline Interspecies Cross.
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DOI：
10.1093/molbev/msab274
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发表时间：
2021-12-09
影响因子：
10.7
通讯作者：
Murphy WJ
中科院分区：
文献类型：
--
作者：
Bredemeyer KR;Seabury CM;Stickney MJ;McCarrey JR;vonHoldt BM;Murphy WJ
种间交配产生的杂种后代的不育性或不成活性是由亲本基因型之间的不亲和性引起的，这种不亲和性被认为是由上位性相互作用中涉及的基因座的分歧引起的。然而，有助于这些区域的快速进化的属性也使它们的组装复杂化，因此候选杂种不育基因座的发现是困难的，并且已被限制于少量的模型系统。在这里，我们报告了快速种间分歧的DXZ 4大卫星位点在种间杂交两个密切相关的哺乳动物物种：家猫（Felis silvestris卡图斯）和丛林猫（Felis chaus）。DXZ4是一个有趣的候选人，由于其结构的复杂性，拷贝数的可变性，并在X染色体失活的关键而复杂的生物过程中的作用。然而，由于其重复的复杂性，DXZ4的完整结构在几乎所有可用的哺乳动物基因组组装中都不存在或不完整。我们比较了三种猫的高度连续的基因组，每种猫都包含一个完整的DXZ4基因座，并发现猫科动物DXZ4基因座与人类直系同源物有很大不同，并且猫物种之间的拷贝数不同。此外，我们报告了DXZ4和X染色体在精子发生阶段的表达，甲基化和结构构象概况，以前与杂交雄性不育有关。总的来说，这些研究结果表明DXZ4在雄性减数分裂中的新作用和通过快速卫星分歧导致猫科动物种间不相容的机制。
The sterility or inviability of hybrid offspring produced from an interspecific mating result from incompatibilities between parental genotypes that are thought to result from divergence of loci involved in epistatic interactions. However, attributes contributing to the rapid evolution of these regions also complicates their assembly, thus discovery of candidate hybrid sterility loci is difficult and has been restricted to a small number of model systems. Here we reported rapid interspecific divergence at the DXZ4 macrosatellite locus in an interspecific cross between two closely related mammalian species: the domestic cat (Felis silvestris catus) and the Jungle cat (Felis chaus). DXZ4 is an interesting candidate due to its structural complexity, copy number variability, and described role in the critical yet complex biological process of X-chromosome inactivation. However, the full structure of DXZ4 was absent or incomplete in nearly every available mammalian genome assembly given its repetitive complexity. We compared highly continuous genomes for three cat species, each containing a complete DXZ4 locus, and discovered that the felid DXZ4 locus differs substantially from the human ortholog, and that it varies in copy number between cat species. Additionally, we reported expression, methylation, and structural conformation profiles of DXZ4 and the X chromosome during stages of spermatogenesis that have been previously associated with hybrid male sterility. Collectively, these findings suggest a new role for DXZ4 in male meiosis and a mechanism for feline interspecific incompatibility through rapid satellite divergence.
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影响因子：
12.3
作者：
Akalin A;Kormaksson M;Li S;Garrett-Bakelman FE;Figueroa ME;Melnick A;Mason CE
通讯作者：
Mason CE
影响因子：
7.2
作者：
Brockdorff, Neil;Turner, Bryan M.
通讯作者：
Turner, Bryan M.
影响因子：
3.3
作者：
Campbell, Polly;Good, Jeffrey M.;Nachman, Michael W.
通讯作者：
Nachman, Michael W.
DOI：
10.1186/s12711-015-0105-9
发表时间：
2015-04-17
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Beissinger TM;Rosa GJ;Kaeppler SM;Gianola D;de Leon N
通讯作者：
de Leon N
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS